Expression of the glucose transporter HXT1 involves the Ser-Thr protein phosphatase Sit4 in Saccharomyces cerevisiae
| Autor: | Michel Ghislain, Suelene F. da Silva, Michel N. Miranda, Mónica Montero-Lomelí, Andréa de Almeida Souza, Bruno L. Bozaquel-Morais, Claudio A. Masuda |
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| Rok vydání: | 2012 |
| Předmět: |
Threonine
Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Phosphatase Immunoblotting Glucose Transport Proteins Facilitative Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Ribosome Peptide Initiation Factors Casein Kinase I Gene Expression Regulation Fungal Protein biosynthesis Protein Phosphatase 2 Strain (chemistry) biology Glucose transporter General Medicine biology.organism_classification Molecular biology Glucose Biochemistry Eukaryotic Initiation Factor-4F Polyribosomes Fermentation |
| Zdroj: | FEMS yeast research. 12(8) |
| ISSN: | 1567-1364 |
| Popis: | We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzing the known essential pathway necessary to induce Hxt1p, we observed a partial inhibition of casein kinase I activity. In both WT and sit4Δ strains, the transcript was induced with no significant difference at 15 min of glucose induction; however, after 45 min, a clear difference in the level of expression was observed being 45% higher in WT than in sit4Δ strain. As at early time of induction, the HXT1 transcript was present but not the protein in the sit4Δ strain we analyzed association of HXT1 with ribosomes, which revealed a significant difference in the association profile; in the mutant strain, the HXT1 transcript associated with a larger set of ribosomal fractions than it did in the WT strain, suggesting also a partial defect in protein synthesis. Overexpression of the translation initiation factor TIF2/eIF4A led to an increase in Hxt1p abundance in the WT strain only. It was concluded that Sit4p ensures that HXT1 transcript is efficiently transcribed and translated thus increasing protein levels of Hxt1p when high glucose levels are present. © 2012 Federation of European Microbiological Societies. |
| Databáze: | OpenAIRE |
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