| Autor: |
Robert, Jérôme, Button, Emily, Stukas, Sophie, Boyce, Guilaine, Ebrima Gibbs, Cowan, Catherine, Gilmour, Megan, Cheng, Wai, Soo, Sonja, Yuen, Brian, Bahrabadi, Arvin, Kang, Kevin, Kulic, Iva, Francis, Gordon, Cashman, Neil, Wellington, Cheryl |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3860566_d1.v1 |
| Popis: |
Aβ induced dose dependent PBMC adhesion and HDL attenuate dose dependent Aβ-induced PBMC adhesion to hCMEC/D3. In all conditions, hCMEC/D3 were stimulated with 0–1 μM Aβ40 (light grey) or Aβ42 (dark grey) monomers for 3 h. Fluorescently labelled PBMC were allowed to adhere to (a) Aβ40- or (b) Aβ42- stimulated cells for 3 additional hours. Cells were washed, fixed, and imaged to count adhered PBMC. hCMEC/D3 were primed with increasing doses (25–400 μg/mL) of HDL for 2 h and stimulated with 0.1 μM (c) Aβ40 (light grey) or (d) Aβ42 (dark grey) for 3 h. Fluorescently labelled PBMC were allowed to adhere to stimulated cells for 3 h followed by washing, fixation, imaging, and counting. Graphs represent mean ± SD of adhered PBMC relative to vehicle control from at least 3 independent trials where * p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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