Comparative evaluation of immunochromatographic dipstick test (ICT) rk39, soluble antigen ELISA and IFAT for the sero-diagnosis of visceral leishmaniasis in Morocco
| Autor: | Faiza Sebti, Abderrahim Sadak, Mohamed Lyagoubi, Hajiba Fellah, Aziza Moustachi, Mohamed Rhajaoui, Mostapha Hida, El Bachir Adlaoui, Abdeslam Et-Touys, Fatima Amarir, Fatima Zahraa Tassou, Youssef Bakri, Meryem Mniouil |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Adolescent Veterinary (miscellaneous) 030106 microbiology 030231 tropical medicine Fluorescent Antibody Technique Antigens Protozoan Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Chromatography Affinity Comparative evaluation 03 medical and health sciences 0302 clinical medicine Internal medicine parasitic diseases medicine Humans Serologic Tests Leishmania infantum Child Soluble antigen biology business.industry Leishmaniasis Gold standard (test) Dipstick Leishmania biology.organism_classification medicine.disease Infectious Diseases Visceral leishmaniasis Insect Science Child Preschool Leishmaniasis Visceral Parasitology Female business |
| Zdroj: | Acta tropica. 182 |
| ISSN: | 1873-6254 |
| Popis: | A rapid, sensitive and specific tool for detection of Leishmania infantum infection in Humans would be highly desirable, because it would allow control interventions in endemic areas of visceral leishmaniasis. This study was carried out at the Reference National Laboratory of Leishmaniasis (RNLL) in National Institute of Hygiene (NIH) Morocco, in order to evaluate the diagnostic potential of immunochromatographic dipstick test (ICT) rk39 in Moroccan suspected VL patients. A total of 49 admitted patients with strong clinical suspicion of VL and 40 healthy controls were investigated for the performance of the ICT rk39. Bone marrow smears were examined for microscopic detection of Leishmania amastigotes obtained from the admitted patients. Only PCR and smear positive cases were considered as gold standard as well as confirmed cases of VL. Out of 49 suspected patients, twenty four (48.9%) were found PCR and smear-positive and twenty three (46.9%) were positive for ICT rk39. Voluntary healthy controls, which included twenty persons from the endemic zone and twenty from non-endemic zone of VL, were found all negative for the strip test. The sensitivity in sera was 75% by ELISA and 87.5% by IFAT, compared with 95.8% for ICT rk39. Specificity was 95.8%, with both tests ELISA and IFAT, and 100% by ICT rk39 respectively. Present study findings again reinforce that the ICT rk39 is a simple, reliable and easy-to-perform non-invasive diagnostic tool for visceral leishmaniasis in the endemic area of Morocco. |
| Databáze: | OpenAIRE |
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