A genome-wide association study reveals cytokinin as a major component in the root defense responses against Ralstonia solanacearum
| Autor: | Wolfgang Busch, Roger de Pedro-Jové, Ondřej Novák, Marc Valls, Alejandro Alonso-Díaz, Hannah M Berry, Núria S. Coll, Santosh B. Satbhai, Christian Göschl, Cristiana T. Argueso |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, Austrian Academy of Sciences, Department of Agriculture (US), CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cytokinins Mecanismes de defensa en les plantes Physiology Arabidopsis Plant Science 01 natural sciences chemistry.chemical_compound GWAS Plant defenses Pathogen Genetics Ralstonia solanacearum biology Bacterial wilt food and beverages Salicylic acid Research Papers Phenotype defense Cytokinin salicylic acid cytokinin 03 medical and health sciences Immunity Defense Gene Plant Diseases hormones AcademicSubjects/SCI01210 fungi Àcid salicílic root biology.organism_classification Arrels (Botànica) Hormones Roots (Botany) immune system 030104 developmental biology Immune system chemistry Root Plant—Environment Interactions Immunitat de les plantes Plants immunity Genome-Wide Association Study 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Dipòsit Digital de la UB Universidad de Barcelona Journal of Experimental Botany |
| Popis: | Bacterial wilt caused by the soil-borne pathogen Ralstonia solancearum is economically devastating, with no effective methods to fight the disease. This pathogen invades plants through their roots and colonizes their xylem, clogging the vasculature and causing rapid wilting. Key to preventing colonization are the early defense responses triggered in the host’s root upon infection, which remain mostly unknown. Here, we have taken advantage of a high-throughput in vitro infection system to screen natural variability associated with the root growth inhibition phenotype caused by R. solanacearum in Arabidopsis during the first hours of infection. To analyze the genetic determinants of this trait, we have performed a genome-wide association study, identifying allelic variation at several loci related to cytokinin metabolism, including genes responsible for biosynthesis and degradation of cytokinin. Further, our data clearly demonstrate that cytokinin signaling is induced early during the infection process and cytokinin contributes to immunity against R. solanacearum. This study highlights a new role for cytokinin in root immunity, paving the way for future research that will help in understanding the mechanisms underpinning root defenses. This work was supported by the Spanish Ministry of Economy and Competitiveness grant nos RyC 2014-16158, AGL2016-78002-R, and PID2019-108595RB-I00/AEI/10.13039/501100011033 to NSC, and through the ‘Severo Ochoa Programme for Centres of Excellence in R&D’ (SEV-2015-0533). We also acknowledge financial support from the CERCA Programme/Generalitat de Catalunya, from the Austrian Academy of Sciences through the Gregor Mendel Institute (WB and SBS) and from funding for CTA and HMB by the United States Department of Agriculture (USDA) grant no. COL00781. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). |
| Databáze: | OpenAIRE |
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