Phylogenetic analysis of canine distemper virus in South America clade 1 reveals unique molecular signatures of the local epidemic
| Autor: | Tiago Gräf, Daniel Thompsen Passos, Cláudio Wageck Canal, Vagner Ricardo Lunge, Marcos A.T. Silveira, Aline Makiejczuk, Fernanda Kieling Moreira Lehmann, André Salvador Kazantzi Fonseca, Cristine Dossin Bastos Fischer, Nilo Ikuta |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male 040301 veterinary sciences Demographic history Population Zoology Hemagglutinins Viral Biology Microbiology 0403 veterinary science 03 medical and health sciences Dogs Genetics medicine Animals Carnivore Distemper Clade education Epidemics Molecular Biology Distemper Virus Canine Ecology Evolution Behavior and Systematics Phylogeny education.field_of_study Molecular Epidemiology Phylogenetic tree Molecular epidemiology Canine distemper Bayes Theorem 04 agricultural and veterinary sciences medicine.disease Virology 030104 developmental biology Infectious Diseases Viral phylodynamics Amino Acid Substitution Mutation RNA Viral Female Brazil |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 41 |
| ISSN: | 1567-7257 |
| Popis: | Canine distemper virus (CDV) is a highly contagious pathogen for domestic dogs and several wild carnivore species. In Brazil, natural infection of CDV in dogs is very high due to the large non-vaccinated dog population, a scenario that calls for new studies on the molecular epidemiology. This study investigates the phylodynamics and amino-acid signatures of CDV epidemic in South America by analyzing a large dataset compiled from publicly available sequences and also by collecting new samples from Brazil. A population of 175 dogs with canine distemper (CD) signs was sampled, from which 89 were positive for CDV, generating 42 new CDV sequences. Phylogenetic analysis of the new and publicly available sequences revealed that Brazilian sequences mainly clustered in South America 1 (SA1) clade, which has its origin estimated to the late 1980's. The reconstruction of the demographic history in SA1 clade showed an epidemic expanding until the recent years, doubling in size every nine years. SA1 clade epidemic distinguished from the world CDV epidemic by the emergence of the R580Q strain, a very rare and potentially detrimental substitution in the viral genome. The R580Q substitution was estimated to have happened in one single evolutionary step in the epidemic history in SA1 clade, emerging shortly after introduction to the continent. Moreover, a high prevalence (11.9%) of the Y549H mutation was observed among the domestic dogs sampled here. This finding was associated (p |
| Databáze: | OpenAIRE |
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