The Chd1 chromatin remodeler can sense both entry and exit sides of the nucleosome
| Autor: | Gregory D. Bowman, Elijah Roberts, Jessica Winger, Michelle D. Wang, Ilana M. Nodelman, Ren Ren, Ashok Kumar Patel, Robert F Levendosky, Ming Li, Kyle Horvath |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Protein domain Plasma protein binding Saccharomyces cerevisiae Lac repressor Biology DNA-binding protein 03 medical and health sciences chemistry.chemical_compound Xenopus laevis Protein Domains Genetics Lac Repressors Nucleosome Animals Gene regulation Chromatin and Epigenetics DNA Chromatin Assembly and Disassembly Chromatin Nucleosomes DNA-Binding Proteins 030104 developmental biology chemistry Biophysics Barrier effect Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Chromatin remodelers are essential for establishing and maintaining the placement of nucleosomes along genomic DNA. Yet how chromatin remodelers recognize and respond to distinct chromatin environments surrounding nucleosomes is poorly understood. Here, we use Lac repressor as a tool to probe how a DNA-bound factor influences action of the Chd1 remodeler. We show that Chd1 preferentially shifts nucleosomes away from Lac repressor, demonstrating that a DNA-bound factor defines a barrier for nucleosome positioning. Rather than an absolute block in sliding, the barrier effect was achieved by altered rates of nucleosome sliding that biased redistribution of nucleosomes away from the bound Lac repressor site. Remarkably, in addition to slower sliding toward the LacO site, the presence of Lac repressor also stimulated sliding in the opposite direction. These experiments therefore demonstrate that Chd1 responds to the presence of a bound protein on both entry and exit sides of the nucleosome. This sensitivity to both sides of the nucleosome allows for a faster and sharper response than would be possible by responding to only the entry side, and we speculate that dual entry/exit sensitivity is also important for regularly spaced nucleosome arrays generated by Chd1 and the related ISWI remodelers. |
| Databáze: | OpenAIRE |
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