In vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome
| Autor: | Wim Teughels, Nico Boon, Esteban R. Herrero, Lynette Zaidel, Frederiek-Maarten Kerckhof, Emma Hernandez-Sanabria, Vera Slomka |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
BIOFILMS phenotype microarray lcsh:QR1-502 DISEASE lcsh:Microbiology Original Research biology FLOW-CYTOMETRY STREPTOCOCCUS-MUTANS COMMUNITY Infectious Diseases HEALTH BIOFILM Oral Microbiome Life Sciences & Biomedicine Microbiology (medical) DENTAL PLAQUE 030106 microbiology Immunology SEQUENCE Microbiology respiratory activity 03 medical and health sciences DYSBIOSIS medicine multivariate statistical analysis Periodontitis FUSOBACTERIUM-NUCLEATUM PATHOGENS Science & Technology commensal bacteria R-PACKAGE Biofilm Aggregatibacter actinomycetemcomitans Biology and Life Sciences Phenotype microarray biology.organism_classification medicine.disease Streptococcus mutans AGGREGATIBACTER-ACTINOMYCETEMCOMITANS MODEL health-associated bacteria Earth and Environmental Sciences VIRULENCE PERIODONTITIS Fusobacterium nucleatum oral pathobionts Bacteria |
| Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, Vol 7 (2017) FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY |
| ISSN: | 2235-2988 |
| DOI: | 10.3389/fcimb.2017.00235 |
| Popis: | Understanding the driving forces behind the shifts in the ecological balance of the oral microbiota will become essential for the future management and treatment of periodontitis. As the use of competitive approaches for modulating bacterial outgrowth is unexplored in the oral ecosystem, our study aimed to investigate both the associations among groups of functional compounds and the impact of individual substrates on selected members of the oral microbiome. We employed the Phenotype Microarray high-throughput technology to analyse the microbial cellular phenotypes of 15 oral bacteria. Multivariate statistical analysis was used to detect respiratory activity triggers and to assess similar metabolic activities. Carbon and nitrogen were relevant for the respiration of health-associated bacteria, explaining competitive interactions when grown in biofilms. Carbon, nitrogen, and peptides tended to decrease the respiratory activity of all pathobionts, but not significantly. None of the evaluated compounds significantly increased activity of pathobionts at both 24 and 48 h. Additionally, metabolite requirements of pathobionts were dissimilar, suggesting that collective modulation of their respiratory activity may be challenging. Flow cytometry indicated that the metabolic activity detected in the Biolog plates may not be a direct result of the number of bacterial cells. In addition, damage to the cell membrane may not influence overall respiratory activity. Our methodology confirmed previously reported competitive and collaborative interactions among bacterial groups, which could be used either as marker of health status or as targets for modulation of the oral environment. ispartof: Frontiers in Cellular and Infection Microbiology vol:7 issue:JUN pages:235- ispartof: location:Switzerland status: published |
| Databáze: | OpenAIRE |
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