Stratification of aggressive prostate cancer from indolent disease—Prospective controlled trial utilizing expression of 11 genes in apparently benign tissue
| Autor: | Esa Kähkönen, Terhi Tallgren, Saeid Alinezhad, Peter J. Boström, Otto Ettala, Kari T. Syvänen, Markku Kallajoki, Pekka Taimen, Ileana Montoya Perez, Kim Pettersson, Hannu J. Aronen, Riina-Minna Väänänen, Matthias Nees, Ivan Jambor |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine PCA3 Oncology medicine.medical_specialty Oncogene Proteins Fusion Urology Cell Cycle Proteins ta3111 urologic and male genital diseases 03 medical and health sciences Prostate cancer 0302 clinical medicine Prostate Internal medicine Biopsy medicine Humans Prospective Studies RNA Messenger Prospective cohort study Aged Aged 80 and over ta3126 medicine.diagnostic_test business.industry Prostatic Neoplasms Cancer Middle Aged Prostate-Specific Antigen ta3122 medicine.disease Magnetic Resonance Imaging Prostate-specific antigen 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Neoplasm Grading Carrier Proteins business Primary Gleason Pattern |
| Zdroj: | Urologic Oncology: Seminars and Original Investigations. 34:255.e15-255.e22 |
| ISSN: | 1078-1439 |
| Popis: | Background: The aim of the study was to evaluate the diagnostic power of molecular markers in men with a clinical suspicion of prostate cancer (PCa) using apparently benign areas as targeted by magnetic resonance imaging (MRI). Methods: In the study, 99 consecutive men with clinical suspicion of PCa in a prospective controlled trial (IMPROD, NCT01864135) were included. In addition to 12-core systematic and MRI-targeted biopsies, cores from normal-appearing prostate areas, based on clinical examination, ultrasound, and biparametric prostate MRI, were obtained. The RNA transcript levels of ACSM1, AMACR, CACNA1D, DLX1, KLK3, PCA3, PLA2G7, RHOU, SPINK1, SPON2, TMPRSS2-ERG, and TDRD1 were measured with quantitative reverse-transcription polymerase chain reaction. Results: Of the 99 men, 69 were diagnosed with PCa, 31 with primary Gleason pattern 3 and 38 with primary Gleason 4 or 5. TDRD1 messenger RNA (mRNA) levels were 1.3 times higher (P = 0.029) and the presence of TMPRSS2-ERG mRNAs more frequent in biopsies from men diagnosed with PCa (27/69, 39%) than in men without (5/30, 16%) (P = 0.035). The 2 markers identified aggressive PCa defined as Gleason sum≥7 at biopsy: median TDRD1 mRNA level was 1.4 higher (P = 0.005) and TMPRSS2-ERG expression more frequent (P |
| Databáze: | OpenAIRE |
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