Measurement of bacterial volume by transmission-through-dye imaging
| Autor: | Moumita Moitra, Michael A. Model, Laura Gunn Leff, Mariana Pelts, Suzanne L. Lababidi |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Microscopy Microscope Materials science Bacteria Staining and Labeling Coloring agents Analytical chemistry Cells Immobilized Microbiology law.invention Transmission (telecommunications) Volume (thermodynamics) law Cell shape Coloring Agents Molecular Biology Biomedical engineering |
| Zdroj: | Journal of microbiological methods. 87(3) |
| ISSN: | 1872-8359 |
| Popis: | Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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