Expression signature of miRNAs and the potential role of miR-195-5p in high-glucose-treated rat cardiomyocytes
| Autor: | Cai-Chuan Yan, Xin Zhao, Yuan-Qi Shi, Mingzhu Li, Yang Li, Guocui Zhang, Yuhao Zhang, Baoxin Li |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male ERG1 Potassium Channel Microarray Diabetic Cardiomyopathies Health Toxicology and Mutagenesis Nedd4 Ubiquitin Protein Ligases hERG Endogeny Protein Serine-Threonine Kinases Toxicology Transfection Biochemistry Immediate-Early Proteins Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Diabetic cardiomyopathy microRNA medicine Animals Humans Myocytes Cardiac Molecular Biology Gene 3' Untranslated Regions Binding Sites 030102 biochemistry & molecular biology biology Mechanism (biology) General Medicine medicine.disease Cell biology Rats MicroRNAs Glucose HEK293 Cells 030220 oncology & carcinogenesis Gene Knockdown Techniques SGK1 biology.protein Molecular Medicine Female Transcriptome |
| Zdroj: | Journal of biochemical and molecular toxicologyREFERENCES. 34(2) |
| ISSN: | 1099-0461 |
| Popis: | MicroRNAs are endogenous small noncoding RNAs that posttranscriptionally regulate the expression of target genes and play crucial roles in diverse physiopathologic processes. In the current study, we examined the microRNA (miRNA) expression profile of high-glucose-treated neonatal rat cardiomyocytes and the potential mechanisms. Differentially expressed miRNAs were analyzed by a miRNA microarray and validated by a quantitative real-time polymerase chain reaction in high-glucose-treated rat cardiomyocytes. Based on the results of our previous study and the bioinformatics prediction, we identified miR-195-5p/SGK1/Nedd4-2/hERG as the top-ranked signal pathway in diabetes cell model in vitro. In summary, our present study provides novel insights into the regulatory mechanism of miR-195-5p/SGK1/Nedd4-2/hERG in rat cardiomyocytes under high-glucose stress, which may provide a novel idea for the development of diagnostic and therapeutic strategies for diabetic cardiomyopathy in the future. |
| Databáze: | OpenAIRE |
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