Chemosensitization of Cancer Cells by KU-0060648, a Dual Inhibitor of DNA-PK and PI-3K
| Autor: | Celine Cano, Pia Thommes, Niall M. B. Martin, Yan Zhao, Liam Cornell, Michael A. Batey, Helen Jenkins, Jody Barbeau, Keith Allan Menear, Julia Bardos, Nicola J. Curtin, Michele Tavecchio, David R. Newell, Roger J. Griffin, Caroline Richardson, Graeme C. M. Smith, Andrew Slade, Joanne M. Munck |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Antineoplastic Agents DNA-Activated Protein Kinase Thiophenes Article Mice Chemosensitization Cell Line Tumor Neoplasms medicine Animals Humans Cytotoxic T cell Enzyme Inhibitors Cytotoxicity Etoposide Phosphoinositide-3 Kinase Inhibitors biology Chemistry Cell growth Topoisomerase fungi Xenograft Model Antitumor Assays Molecular biology Tumor Burden Enzyme Activation enzymes and coenzymes (carbohydrates) Oncology Chromones Drug Resistance Neoplasm Cell culture Cancer cell Immunology MCF-7 Cells biology.protein Female biological phenomena cell phenomena and immunity medicine.drug |
| Zdroj: | Molecular Cancer Therapeutics. 11:1789-1798 |
| ISSN: | 1538-8514 1535-7163 |
| Popis: | DNA double-strand breaks (DSB) are the most cytotoxic lesions induced by topoisomerase II poisons. Nonhomologous end joining (NHEJ) is a major pathway for DSB repair and requires DNA-dependent protein kinase (DNA-PK) activity. DNA-PK catalytic subunit (DNA-PKcs) is structurally similar to PI-3K, which promotes cell survival and proliferation and is upregulated in many cancers. KU-0060648 is a dual inhibitor of DNA-PK and PI-3K in vitro. KU-0060648 was investigated in a panel of human breast and colon cancer cells. The compound inhibited cellular DNA-PK autophosphorylation with IC50 values of 0.019 μmol/L (MCF7 cells) and 0.17 μmol/L (SW620 cells), and PI-3K–mediated AKT phosphorylation with IC50 values of 0.039 μmol/L (MCF7 cells) and more than 10 μmol/L (SW620 cells). Five-day exposure to 1 μmol/L KU-0060648 inhibited cell proliferation by more than 95% in MCF7 cells but only by 55% in SW620 cells. In clonogenic survival assays, KU-0060648 increased the cytotoxicity of etoposide and doxorubicin across the panel of DNA-PKcs–proficient cells, but not in DNA-PKcs–deficient cells, thus confirming that enhanced cytotoxicity was due to DNA-PK inhibition. In mice bearing SW620 and MCF7 xenografts, concentrations of KU-0060648 that were sufficient for in vitro growth inhibition and chemosensitization were maintained within the tumor for at least 4 hours at nontoxic doses. KU-0060648 alone delayed the growth of MCF7 xenografts and increased etoposide-induced tumor growth delay in both in SW620 and MCF7 xenografts by up to 4.5-fold, without exacerbating etoposide toxicity to unacceptable levels. The proof-of-principle in vitro and in vivo chemosensitization with KU-0060648 justifies further evaluation of dual DNA-PK and PI-3K inhibitors. Mol Cancer Ther; 11(8); 1789–98. ©2012 AACR. |
| Databáze: | OpenAIRE |
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