Bone regeneration in a rabbit ulna defect model: use of allogeneic adipose-derivedstem cells with low immunogenicity
| Autor: | Chen Wang, Ni Mei, Zhuyou Xiong, Guangzao Li, Bing Li, Xiaofan Yin, Huijie Gu |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Bone Regeneration Time Factors Histology Cell Adipose tissue Ulna Pathology and Forensic Medicine Imaging Three-Dimensional Tissue engineering Osteogenesis Animals Transplantation Homologous Medicine Cell Lineage Bone regeneration Cell Shape Cells Cultured Cell Proliferation Immunosuppression Therapy business.industry Demineralized bone matrix Stem Cells Immunogenicity Mesenchymal stem cell Cell Differentiation hemic and immune systems Cell Biology Anthozoa Extracellular Matrix Surgery Disease Models Animal medicine.anatomical_structure Adipose Tissue Antigens Surface Cancer research Rabbits Lymphocyte Culture Test Mixed Stem cell Tomography X-Ray Computed business Stem Cell Transplantation |
| Zdroj: | Cell and Tissue Research. 358:453-464 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-014-1952-3 |
| Popis: | Tissue engineering provides new potential treatments for the repair of bone defects. Bone-marrow-derived mesenchymal stem cells (BMSCs) represent an attractive cell source for therapeutic applications involving tissue engineering, although disadvantages, such as pain of harvest and low proliferation efficiency, are major limitations to the application of BMSCs in the clinic. Adipose-derived stem cells (ASCs) with their multilineage potential and satisfactory proliferation potential can be induced into the osteogenic lineage in vitro and can be anchored onto suitable scaffolds as seed cells to repair bone defects successfully in an autologous setting. Previous studies have indicated that both undifferentiated BMSCs and ASCs exhibit immunosuppression and immunoprivilege properties. We compare the immuno-function of undifferentiated and osteo-differentiated ASCs in vitro and explore the feasibility of applying allogeneic ASCs to the repair of ulnar bone defects in the rabbit model. Our study demonstrates that allogeneic osteogenic differentiated ASCs maintain low immunogenicity and negative immunomodulation. The allogeneic osteogenic differentiated ASCs combined with demineralized bone matrix successfully regenerate ulnar bone defects in rabbits without immunosuppressive therapies. |
| Databáze: | OpenAIRE |
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