Use of glycogen phosphorylase BB measurement with POCT in the diagnosis of acute coronary syndromes. A comparison with the ELISA method
| Autor: | Pavlina Solichova, Libor Jedelsky, Petra Seitlova, Michal Karpisek, Ludek Sprongl, Jitka Proskova, Borek Lacnak, Marie Janosova, David Stejskal, Lenka Kadalova, Leona Stepanova |
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| Rok vydání: | 2007 |
| Předmět: |
Proband
medicine.medical_specialty Acute coronary syndrome Point-of-Care Systems Point-of-care testing Myocardial Infarction Enzyme-Linked Immunosorbent Assay Chest pain Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Glycogen phosphorylase Internal medicine medicine Humans cardiovascular diseases Myocardial infarction Acute Coronary Syndrome Elisa method business.industry Glycogen Phosphorylase medicine.disease Isoenzymes Elisa test Cardiology Reagent Kits Diagnostic medicine.symptom business Biomarkers |
| Zdroj: | ResearcherID |
| ISSN: | 1804-7521 1213-8118 |
| DOI: | 10.5507/bp.2007.041 |
| Popis: | BACKGROUND Glycogen Phosphorylase BB (GPBB) is considered an early and specific marker of myocardial necrosis and ischemia. A POCT kit GPBB for diagnostic use has recently been approved. AIM an evaluation of the correspondence of qualitative POCT GBPP measurements with ELISA test results. MATERIAL AND METHODOLOGY 20 individuals with non-ST elevation myocardial infarction (non-STEMI) and 20 probands without acute coronary syndrome (ACS) were tested. GPBB (POCT, ELISA) in venous plasma (lithium-heparin) was assayed in all probands. RESULTS individuals with non-STEMI had significantly higher GPBB ELISA values (32.3 vs. 6.1 microg/l; p < 0.01). GPBB sensitivity and specificity for non-STEMI presence 6 hours after chest pain generation were 100 %. No proband was classified in a different subgroup with POCT of GPBB (positive/negative). GPBB POCT correlate with a non- STEMI diagnosis (chi(2) 36.1; p |
| Databáze: | OpenAIRE |
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