Regeneration pattern of precursor-B-cells in bone marrow of acute lymphoblastic leukemia patients depends on the type of preceding chemotherapy
| Autor: | Herbert Hooijkaas, J. J. M. Van Dongen, Karel Hählen, E. G. Van Lochem, Y. M. Wiegers, R.W.M. van den Beemd |
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| Přispěvatelé: | Immunology, Pediatrics |
| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Neoplasm Residual Childhood leukemia Dexamethasone Immunophenotyping Andrology DNA Nucleotidylexotransferase hemic and lymphatic diseases Acute lymphocytic leukemia Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Antineoplastic Combined Chemotherapy Protocols medicine Biomarkers Tumor Neoplasm Asparaginase Humans Child B-Lymphocytes Microscopy business.industry Regeneration (biology) Cell Differentiation Hematology medicine.disease Flow Cytometry Hematopoietic Stem Cells Hematopoiesis Neoplasm Proteins Leukemia medicine.anatomical_structure Oncology Terminal deoxynucleotidyl transferase Vincristine Neoplastic Stem Cells Drug Evaluation Female Neprilysin Bone marrow business Cell Division |
| Zdroj: | Leukemia, 14, 688-695. Nature Publishing Group |
| ISSN: | 0887-6924 |
| Popis: | Immunofluorescence stainings for the CD10 antigen and terminal deoxynucleotidyl transferase (TdT) can be used for the detection of leukemic blasts in CD10+ precursor-B-acute lymphoblastic leukemia (precursor-B-ALL) patients, but can also provide insight into the regeneration of normal precursor-B-cells in bone marrow (BM). Over a period of 15 years, we studied the regeneration of CD10+, TdT+, and CD10+/TdT+ cells in BM of children with (CD10+) precursor-B-ALL during and after treatment according to three different treatment protocols of the Dutch Childhood Leukemia Study Group (DCLSG) which differed both in medication and time schedule. This study included a total of 634 BM samples from 46 patients who remained in continuous complete remission (CCR) after treatment according to DCLSG protocols VI (1984-1988; n = 8), VII (1988-1991; n = 10) and VIII (1991-1997; n = 28). After the cytomorphologically defined state of complete remission with CD10+ and CD10+/TdT+ frequencies generally below 1% of total BM cells, a 10-fold increase in precursor-B-cells was observed in protocol VII and protocol VIII, but not in protocol VI. At first sight this precursor-B-cell regeneration during treatment resembled the massive regeneration of the precursor-B-cell compartment after maintenance treatment, and appeared to be related to the post-induction or post-central nervous system (CNS) therapy stops in protocols VII and VIII. However, careful evaluation of the distribution between the 'more mature' (CD10+/TdT-) and the 'immature' (CD10+/TdT+) precursor-B-cells revealed major differences between the post-induction/post-re-induction precursor-B-cell regeneration (low 'mature/immature' ratio: generally |
| Databáze: | OpenAIRE |
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