Biogenic synthesis of silver nanoparticles: Antibacterial and cytotoxic potential
| Autor: | Mudawi M. Elobeid, Asma S. Algebaly, Afrah E. Mohammed, Nael Abutaha |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Green chemistry Reducing agent Cytotoxicity Nanoparticle medicine.disease_cause 01 natural sciences Silver nanoparticle Article 03 medical and health sciences medicine Escherichia coli lcsh:QH301-705.5 Azadirachta indica Chemistry Calligonum comosum Antimicrobial AgNPs Antibacterial 030104 developmental biology lcsh:Biology (General) Transmission electron microscopy Caspase 3/7 General Agricultural and Biological Sciences 010606 plant biology & botany Nuclear chemistry |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 5, Pp 1340-1351 (2020) Saudi Journal of Biological Sciences |
| Popis: | In green chemistry, the application of a biogenic material as a mediator in nanoparticles formation is an innovative nanotechnology. Our current investigation aimed at testing the cytotoxic potential and antimicrobial ability of silver nanoparticles (AgNPs) that were prepared using Calligonum comosum roots and Azadirachta indica leaf extracts as stabilizing and reducing agents. An agar well diffusion technique was employed to detect synthesized AgNPs antibacterial ability on Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus bacterial strains. Furthermore, their cytotoxic capability against LoVo, MDA-MB231 and HepG2 ca cells was investigated. For phyto-chemical detection in the biogenic AgNPs the Fourier-transform infrared spectroscopy (FT-IR) was considered. Zeta sizer, TEM (Transmission Electron Microscope) and FE-SEM (Field Emission Scanning Electron Microscope) were used to detect biogenic AgNPs’ size and morphology. The current results showed the capability of tested plant extract for conversion of Ag ions to AgNPs with a mean size ranging between 90.8 ± 0.8 and 183.2 ± 0.7 nm in diameter. Furthermore, prepared AgNPs exhibited apoptotic potential against HepG2, LoVo, and MDA-MB 231cell with IC50 ranging between 10.9 and 21.4 μg/ml and antibacterial ability in the range of 16.0 ± 0.1 to 22.0 ± 1.8 mm diameter. Activation of caspases in AgNPs treated cells could be the main indicator for their positive effect causing apoptosis. The current investigation suggested that the green production of AgNPs could be a suitable substitute to large-scale production of AgNPs, since stable and active nanoparticles could be obtained. Keywords: AgNPs, Calligonum comosum, Azadirachta indica, Cytotoxicity, Caspase 3/7, Antibacterial |
| Databáze: | OpenAIRE |
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