Chemico-mechanical imaging of Barrett's oesophagus

Autor: Jayakrupakar Nallala, Francesca Palombo, Hugh Barr, Nicholas Stone, Antoine David, Marco Madami, Daniele Fioretto
Rok vydání: 2016
Předmět:
Genetics and Molecular Biology (all)
Pathology
medicine.medical_specialty
Esophageal Neoplasms
General Physics and Astronomy
Oesophageal biopsy
02 engineering and technology
Adenocarcinoma
Spectrum Analysis
Raman
Biochemistry
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
Epithelium
010309 optics
Physics and Astronomy (all)
symbols.namesake
Barrett Esophagus
Engineering (all)
0103 physical sciences
Microscopy
Spectroscopy
Fourier Transform Infrared
medicine
Humans
Biomechanics
General Materials Science
Raman
Chemistry
Brillouin
Chemistry (all)
General Engineering
Light scattering
General Chemistry
021001 nanoscience & nanotechnology
medicine.disease
digestive system diseases
FTIR spectroscopy
medicine.anatomical_structure
Epithelial tissue
Barrett's oesophagus
symbols
Materials Science (all)
Esophagoscopy
0210 nano-technology
Raman spectroscopy
Biochemistry
Genetics and Molecular Biology (all)
Zdroj: Journal of biophotonics. 9(7)
ISSN: 1864-0648
Popis: Barrett's oesophagus is a condition characterized by a change in the lining of the oesophagus that markedly increases the risk of adenocarcinoma. We demonstrate the first site-matched application of Brillouin microscopy, Raman microscopy and FTIR micro-spectroscopic imaging to ex-vivo epithelial tissue - Barrett's oesophagus. The mechanical and chemical characters of the epithelium were assessed in histological sections from a patient subjected to endoscopic oesophageal biopsy. Previous studies have shown that both these properties change within the oesophageal wall, owing to the presence of distinct cellular and extracellular constituents which are putatively affected by oesophageal cancer. Brillouin microscopy enables maps of elasticity of the epithelium to be obtained, whilst Raman and FTIR imaging provide 'chemical images' without the need for labelling or staining. This site-matched approach provides a valuable platform for investigating the structure, biomechanics and composition of complex heterogeneous systems. A combined Brillouin-Raman device has potential for in-vivo diagnosis of pathology. First application of site-matched micro Brillouin, Raman and FTIR spectroscopic imaging to epithelial tissue in Barrett's oesophagus.
Databáze: OpenAIRE
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