Agalactosyl IgG in aggregates from the rheumatoid joint
| Autor: | Christopher J. Elson, G. C. Lastra, K. A. Leader, John R. Kirwan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Glycosylation
biology business.industry Polyethylene glycol Immunoglobulin G Polyethylene Glycols Arthritis Rheumatoid chemistry.chemical_compound Rheumatology Biochemistry chemistry Galactose Lectins PEG ratio Synovial Fluid biology.protein Synovial fluid Medicine Humans Pharmacology (medical) Protein G Antibody business |
| Zdroj: | British journal of rheumatology. 35(4) |
| ISSN: | 0263-7103 |
| Popis: | SUMMARY It has been postulated that agalactosyl immunoglobulin G (IgG) self-associates to form pathological aggregates in the rheumatoid joint. To examine this hypothesis, IgG aggregates from synovial fluid (SF) of 22 patients with RA were prepared by precipitation with polyethylene glycol (PEG) 6000. The PEG precipitates and SFs were reduced with 2-mercaptoethanol (2ME) and bound to protein G. This procedure isolated the IgG in the PEG precipitates from other contaminating glycosylated proteins. The levels of galactose and A^-acetylglucosamine (GlcNAc) residues present on the reduced IgG were quantified by their ability to bind the lee tins Ricinus communis (RCA)i» and Bandeiraea simplicifolia (BS) II. Proportionally less galactose (expressed as a ratio of bound RCAis to BS IT) was present on the IgG from the PEG precipitates than on the IgG in the paired SF (P = 0.001). However, in many cases more RCA,» as well as BS II bound to IgG from PEG precipitates than from the corresponding SF. It is considered that agalactosyl IgG occurs preferentially in RA SF PEG precipitates and that this IgG may also exhibit increased Fab glycosylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|