Neuropeptide-Induced Mast Cell Degranulation and Characterization of Signaling Modulation in Response to IgE Conditioning
| Autor: | Sarah M. Gruba, Benjamin M. Manning, Christy L. Haynes, A. Meyer |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cell Degranulation Calcitonin gene-related peptide Immunoglobulin E Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Animals Mast Cells Neurogenic inflammation biology Neuropeptides Degranulation General Medicine Mast cell Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Immunology biology.protein Molecular Medicine Signal transduction Microelectrodes 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | ACS chemical biology. 11(11) |
| ISSN: | 1554-8937 |
| Popis: | As tissue-resident immune cells, mast cells are frequently found in close proximity to afferent neurons and are subjected to immunoactive mediators secreted by these neurons, including substance P (SP) and calcitonin gene-related peptide (CGRP). Neurogenic inflammation is thought to play an important role in the pathophysiology of many diseases. Unraveling the cellular mechanisms at the interface between the immune response and the peripheral nervous system is important for understanding how these diseases arise and progress. In this work, mast cell degranulation following direct exposure to CGRP and SP was studied both at the bulk and single-cell levels to characterize the mouse peritoneal mast cell response to neuropeptides and compare this response to well-studied mast cell activation pathways. Results show that mast cells secrete fewer chemical messenger-filled granules with increased IgE preincubation concentrations. The biophysical characteristics of mast cell degranulation in response to SP and CGRP is in many ways similar to calcium ionophore-induced mast cell degranulation; however, neuropeptide-stimulated mast cells secrete reduced chemical messenger content per secretion event, resulting in an overall relative decrease in secreted chemical messengers. |
| Databáze: | OpenAIRE |
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