| Popis: |
The influenza virus strains PR8, FM1, and Lee were fractionated with the anion exchange resin Amberlite IRA-400 using a salt concentration gradient elution method. Strain PR8 showed three distinct peaks of hemagglutinating and infective virus in the diagram of chromatography. The first peak (No. I) was due to a virus fraction not adsorbed by the anion exchange resin. The other two virus peaks (Nos. IV and VIII) were obtained by elution from the resin with salt solution of increasing concentration. In studies with strain FM1 only two activity peaks were found, the first being attributable to a nonadsorbed fraction, while the other peak resulted from elution with a salt solution intermediate in concentration between the solutions producing the second (No. IV) and third peaks (No. VIII) of PR8. The Lee strain, in turn, yielded only one peak in repeated tests. The virus was not adsorbed on the anion exchange resin and appeared to be uniform under the experimental conditions used. Serological examination of the three fractions of PR8, using the hemagglutination-inhibition, complement-fixation, and egg neutralization tests showed significant differences only with regard to hemagglutination-inhibition. Adsorption and elution of PR8-infected allantoic fluid with chicken red cells yielded two distinct fractions, A and B, which could be differentiated by ion exchange chromatography. The bulk of fraction A remained in the supernatant after treatment with the cells, but a small portion was also present in the eluates obtained from them. Fraction B was almost completely adsorbed by the cells and could be eluted in full amount. It was shown by chromatography tests that fraction A corresponds physicochemically to fraction I and fraction B to fraction IV. A third fraction (C) remained in the supernatant after treatment with erythrocytes. It was not detected in the eluates from the cells and, in anion exchange chromatography, behaved differently from fraction VIII in that it could be eluted from the column only by salt solution of high concentration and by raising the pH from 6.0 to 7.5. It appears likely, therefore, that fraction VIII, demonstrable by chromatography only, was transformed into fraction C by treatment with the cells. |
