Diversity within Aspergillus niger Clade and Description of a New Species: Aspergillus vinaceus sp. nov
| Autor: | Olivier Puel, Maria Helena Pelegrinelli Fungaro, Larissa de Souza Ferranti, Marta Hiromi Taniwaki, Sophie Lorber, Beatriz T. Iamanaka, Jens Christian Frisvad, Josué José da Silva, Fernanda Pelisson Massi |
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| Přispěvatelé: | State University of Londrina = Universidade Estadual de Londrina, Instituto de Tecnologia de Alimentos (ITAL), Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Biotechnology and Biomedicine, Technical University of Denmark [Lyngby] (DTU), This research was funded by: Conselho Nacional de Pesquisa (CNPq, research fellowship awarded to M.H.P.F. and M.H.T. (CNPq 310970/2015-6 and CNPq 303732/2018-0), Coordenadoria de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES, scholarship awarded to J.J.S.—Finance Code 001), Fundação de Amparo a Pesquisa do Estado |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Aspergillus niger clade Sclerotium Plant Science Genetic diversity 03 medical and health sciences chemistry.chemical_compound parasitic diseases Clade GCPSR lcsh:QH301-705.5 Gene Ecology Evolution Behavior and Systematics [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology 030304 developmental biology Genetics genealogical concordance 0303 health sciences Aspergillus biology 030306 microbiology secondary metabolites Secondary metabolites GCSPR Haplotype Aspergillus niger genetic diversity biology.organism_classification lcsh:Biology (General) chemistry Genealogical concordance DNA |
| Zdroj: | Journal of Fungi Volume 6 Issue 4 Journal of Fungi, MDPI, 2020, 6 (4), pp.371. ⟨10.3390/jof6040371⟩ da Silva, J J, Iamanaka, B T, Ferranti, L S, Massi, F P, Taniwaki, M H, Puel, O, Lorber, S, Frisvad, J C & Fungaro, M H P 2020, ' Diversity within Aspergillus niger Clade and Description of a New Species: Aspergillus vinaceus sp. nov ', Journal of Fungi, vol. 6, no. 4, 371 . https://doi.org/10.3390/jof6040371 Journal of Fungi, Vol 6, Iss 371, p 371 (2020) |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof6040371 |
| Popis: | Diversity of species within Aspergillus niger clade, currently represented by A. niger sensu stricto and A. welwitshiae, was investigated combining three-locus gene sequences, Random Amplified Polymorphic DNA, secondary metabolites profile and morphology. Firstly, approximately 700 accessions belonging to this clade were investigated using calmodulin gene sequences. Based on these sequences, eight haplotypes were clearly identified as A. niger (n = 247) and 17 as A. welwitschiae (n = 403). However, calmodulin sequences did not provide definitive species identities for six haplotypes. To elucidate the taxonomic position of these haplotypes, two other loci, part of the beta-tubulin gene and part of the RNA polymerase II gene, were sequenced and used to perform an analysis of Genealogical Concordance Phylogenetic Species Recognition. This analysis enabled the recognition of two new phylogenetic species. One of the new phylogenetic species showed morphological and chemical distinguishable features in comparison to the known species A. welwitschiae and A. niger. This species is illustrated and described as Aspergillus vinaceus sp. nov. In contrast to A. niger and A. welwitschiae, A. vinaceus strains produced asperazine, but none of them were found to produce ochratoxin A and/or fumonisins. Sclerotium production on laboratory media, which does not occur in strains of A. niger and A. welwitschiae, and strictly sclerotium-associated secondary metabolites (14-Epi-hydroxy-10,23-dihydro-24,25-dehydroaflavinine 10,23-Dihydro-24,25-dehydroaflavinine 10,23-Dihydro-24,25-dehydro-21-oxo-aflavinine) were found in A. vinaceus. The strain type of A. vinaceus sp. nov. is ITAL 47,456 (T) (=IBT 35556). |
| Databáze: | OpenAIRE |
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