Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea
| Autor: | Mei Wang, Wanru Hou, Changwu Yue, Xiang Ding, Yiling Hou, Yuanxiu Zhu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Antioxidant DPPH Amanita medicine.medical_treatment Pentoses 02 engineering and technology Polysaccharide Methylation Biochemistry 03 medical and health sciences chemistry.chemical_compound Hydrolysis Residue (chemistry) Picrates Polysaccharides Genetics medicine Benzothiazoles Molecular Biology chemistry.chemical_classification ABTS biology Biphenyl Compounds Free Radical Scavengers 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Amanita caesarea Glucose 030104 developmental biology Oncology chemistry Sephadex Molecular Medicine Sulfonic Acids 0210 nano-technology |
| Zdroj: | Molecular Medicine Reports. 14:3947-3954 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2016.5693 |
| Popis: | A heteropolysaccharide was isolated from the fruiting bodies of Amanita caesarea using a diethylaminoethyl-cellulose column, Sephacryl S‑300 gel column and Sephadex G‑200 column. The Amanita caesarea polysaccharide was predominantly composed of α-D-glucose and α-D-lyxose at a ratio of 2:1, and it had a molecular weight of 19,329 Da. The structural features of the Amanita caesarea polysaccharide were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry, and infrared spectra and nuclear magnetic resonance spectroscopy. The results showed that Amanita caesarea polysaccharide (termed AC‑1) had a backbone of 1,4‑linked α‑D‑glucose and 1,3,6‑linked α‑D‑glucose, with branches of one 1‑linked α‑D‑lyxose residue. The antioxidant activity of AC‑1 was evaluated by two biochemical methods, 2,2-azino-bis diammonium (ABTS+) radical scavenging activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH-) radical scavenging activity. The uncontrolled production of free radicals is involved in various diseases, including cancer, atherosclerosis and degenerative aging processes. The results indicated that the Amanita caesarea polysaccharide exhibits strong antioxidant activity, thus, it may be a useful natural product antioxidant. |
| Databáze: | OpenAIRE |
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