Antisense oligodeoxynucleotide against human telomerase reverse transcriptase inhibits the proliferation of Eca-109 esophageal carcinoma cells
| Autor: | Zhou Wang, Xiang‑Kui Fan, Bao‑Jiang Li, Lin Wei, Rui‑Hua Yan, Xiang‑Ming Chen |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Telomerase Oncogene Cell growth telomerase reverse transcriptase esophageal neoplasms Articles General Medicine Cell cycle Biology Molecular biology chemistry.chemical_compound Immunology and Microbiology (miscellaneous) chemistry Cell culture Immunology Telomerase reverse transcriptase MTT assay Growth inhibition antisense oligodeoxynucleotides |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2014.1897 |
| Popis: | Previous studies have demonstrated that the growth of tumor cells may be inhibited by antisense oligonucleotides (ASODNs) targeted against human telomerase (hTR) or human telomerase reverse transcriptase (hTERT), resulting in antitumor activity in a wide variety of tumors. However, few studies have investigated the effect of hTERT gene-targeted ASODNs on telomerase activity and cell proliferation in human esophageal cancer. In the present study, an MTT assay was used to determine the growth inhibition rate of Eca-109 cells treated with a hTERT-targeted phosphorothioate-ASODN (PS-ASODN). An inverted microscope was used to observe the morphologic changes of the cells following treatment with 5 μM PS-ASODN for 10 days. Telomerase activity was detected using the silver staining semi-quantitative telomeric repeat amplification protocol (TRAP) assay. Following treatment with the PS-ASODN (1–5 μmol/l), the proliferation of the Eca-109 cells was inhibited. The differences in inhibition rate between the PS-ASODN and blank control groups were statistically significant (P |
| Databáze: | OpenAIRE |
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