Serological Characterization of Human Monocytes for HLA, B-Lymphocyte, Granulocyte, and Monocyte-Associated Antigens by Cytotoxicity Testing
| Autor: | P. Zamfirescu, B. M. Carter, David S. Nathanson, Paul I. Terasaki, S. Ian Drew, Ronald Billing, Faramarz Naiem |
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| Rok vydání: | 1978 |
| Předmět: |
Isoantigens
T-Lymphocytes Lymphocyte Transplantation Heterologous Immunology Population Histocompatibility Testing Human leukocyte antigen Granulocyte Biology Biochemistry Monocytes Antigen HLA Antigens Genetics medicine Humans Transplantation Homologous Immunology and Allergy Blood Transfusion education Pan-T antigens B-Lymphocytes education.field_of_study Immune Sera Monocyte Complement System Proteins General Medicine Cytotoxicity Tests Immunologic medicine.anatomical_structure Granulocytes |
| Zdroj: | Tissue Antigens. 11:385-393 |
| ISSN: | 1399-0039 0001-2815 |
| Popis: | Highly enriched preparations of monocytes, B and T lymphocytes, and granulocytes from 18 normal donors were serotyped in parallel in a complement-dependent cytotoxicity assay using allogeneic and heterologous antisera defining three independent tissue antigen systems. HLA and B-lymphocyte tissue antigens were detected on human monocytes although granulocyte antigens were absent. By cytotoxicity testing the presence of Ia-like antigens on monocytes was significantly diminished compared to the autologous B-lymphocyte population and has important implications in B-lymphocyte serology. The study indentified a number of human antisera obtained from multitransfused subjects and pre- and post-transplant organ recipients that were non-HLA and appeared to define monocyte-associated antigens. The serological implications of surface antigen expression on human monocytes compared with other peripheral blood cells are discussed. |
| Databáze: | OpenAIRE |
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