Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development
| Autor: | Gerhard Gross, Marion Ahrens, Dietmar Bächner, Dietmar Schröder, Nicole Betat |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Apolipoprotein E
Cellular differentiation Clinical Biochemistry Mice Inbred Strains Biology Transfection Bone morphogenetic protein Biochemistry Bone morphogenetic protein 2 Cell Line Mesoderm Embryonic and Fetal Development Mice Apolipoproteins E medicine Animals Cell Lineage Cloning Molecular Cells Cultured In Situ Hybridization Osteoblasts Stem Cells Mesenchymal stem cell Gene Expression Regulation Developmental Neural crest Cell Differentiation Osteoblast General Medicine Embryo Mammalian Molecular biology Embryonic stem cell Recombinant Proteins Up-Regulation medicine.anatomical_structure Neural Crest Bone Morphogenetic Proteins embryonic structures Molecular Medicine lipids (amino acids peptides and proteins) |
| Zdroj: | BioFactors. 9:11-17 |
| ISSN: | 1872-8081 0951-6433 |
| DOI: | 10.1002/biof.5520090103 |
| Popis: | Apolipoprotein E (ApoE) was identified as upregulated by Bmp-2 (bone morphogenetic protein-2) in the murine mesenchymal progenitor cell line C3H10T1/2 by a subtractive cloning strategy. Expression of recombinant Bmps in mesenchymal C3H10T1/2 progenitors results in the differentiation into the osteogenic, the chondrogenic, and the adipogenic lineage. In addition, ApoE is also expressed in primary osteoblasts isolated from murine calvariae late in the in vitro osteoblast developmental sequence. To infer possible roles of ApoE in organogenesis and tissue differentiation, ApoE expression during mouse embryonic development was analyzed in murine midgestation and late embryonic development by in situ hybridization. ApoE is highly expressed at many sites of organ development (liver, brain, heart, eye, lung), probably in a subset of neural crest cells and ectodermal derivatives suggestive for important functions of ApoE during embryonic differentiation and organ development. |
| Databáze: | OpenAIRE |
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