Hemorrhagic shock induces differential gene expression and apoptosis in mouse liver
| Autor: | Radha K. Maheshwari, Ying-Yue Li, Shirin V. Sundar, Florence M. Rollwagen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Biophysics Gene Expression Apoptosis Cell Cycle Proteins Biology Shock Hemorrhagic Biochemistry Mice Downregulation and upregulation Proto-Oncogene Proteins Gene expression medicine Animals Aspartate Aminotransferases RNA Messenger Molecular Biology Transcription factor Gene Oligonucleotide Array Sequence Analysis Liver injury Mice Inbred BALB C Gadd45 Gene Expression Profiling Nuclear Proteins alpha-Crystallin B Chain Alanine Transaminase Proto-Oncogene Proteins c-mdm2 Cell Biology medicine.disease Molecular biology Gene expression profiling Oxidative Stress Liver Cancer research Female Lipid Peroxidation Immediate early gene Transcription Factors |
| Zdroj: | Biochemical and biophysical research communications. 332(3) |
| ISSN: | 0006-291X |
| Popis: | Comprehensive knowledge of the gene expression changes induced by hemorrhage in vital organs will greatly improve prognosis and therapy. Therefore, we used a mouse model of non-resuscitated hemorrhagic shock to study the pattern of stress-induced genes in liver at 1, 4, and 24 h following surgery. Hepatic injury was confirmed by assessment of liver injury markers and apoptotic cell death. We found that a variety of stress-regulated genes were differentially expressed, including seven genes that have not been reported previously as being regulated by hemorrhagic shock: ATF-2, alphaB-crystallin, GADD45, GADD45beta, Mdm2, p21Waf1, and TRPM-2. The changes in mRNA levels of the transcription factors AP-1, Egr-1, HSF-1, and NF-kappaB were transient but protein expression was noticeable at later time points. Our findings show that oxidative stress causes immediate upregulation of genes involved in a variety of cellular defense pathways. Complex interactions among them might determine the ultimate fate of the cell. |
| Databáze: | OpenAIRE |
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