Modulatory effects of melatonin on genotoxic response of reference mutagens in the Ames test and the comet assay
Autor: | Véronique André, V.N. Anisimov, C. Vigreux, Pascal Gauduchon, S.A. Musatov, T. Godard, François Sichel |
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Rok vydání: | 1998 |
Předmět: |
Salmonella typhimurium
endocrine system Mutagenicity Tests Chemistry Health Toxicology and Mutagenesis Mitomycin C DMBA CHO Cells Molecular biology Antioxidants Ames test Comet assay chemistry.chemical_compound Clastogen Biochemistry Cricetinae Genetics Animals Sodium azide Antimutagen Carcinogen Melatonin Mutagens |
Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 417:75-84 |
ISSN: | 1383-5718 |
DOI: | 10.1016/s1383-5718(98)00094-1 |
Popis: | The effect of a potent endogenous antioxidant, the pineal gland indole melatonin (MLT) on the mutagenicity of twelve well-known mutagens and carcinogens has been investigated using two in vitro tests the Ames test and the single cell gel electrophoresis assay (SCGE assay or COMET assay). The 12 mutagens used were 7,12-dimethylbenz( a )anthracene (DMBA), benzo( a )pyrene (BP), 2-aminofluorene (AF), 1,2-dimethylhydrazine (DMH), bleomycin, cyclophosphamide (CP), 4-nitroquinoline- N -oxide (NQO), 2,4,7-trinitro-9-fluorenone (TNF), 9-aminoacridine (AA), N -nitrosomethylurea (NMU), mitomycin C and sodium azide tested in the absence or in the presence of S9 mix. MLT alone turned out neither toxic nor mutagenic in the Ames test and revealed clastogenic activity at the highest concentration tested (100 μM) in the SCGE assay. In four Salmonella typhimurium tester strains TA 97, TA 98, TA 100 and TA 102 MLT significantly reduced the mutagenicity of chemicals which require S9 activation. In the SCGE assay performed on CHO cells, preincubation with MLT led to a strong inhibition of clastogenic activities of DMBA and CP, and in a lesser extent with BP and NMU. With mitomycin C, MLT exacerbated responses in both tests. The possible mechanisms of MLT's inhibitory action are discussed. |
Databáze: | OpenAIRE |
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