Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
| Autor: | Alexander F. Schober, Serena Flori, Carolina Río Bártulos, Peter G. Kroth, Giovanni Finazzi |
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| Přispěvatelé: | Department of Biology, University of Konstanz, The Laboratory (Marine Biological Association of the United Kingdom), Marine Biological Association of the United Kingdom (MBA), Physiologie cellulaire et végétale (LPCV), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Marechal Eric, Marine Biological Association of the United Kingdom, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Endosymbiosis Chemistry Thalassiosira pseudonana Complex plastid fractionation biology.organism_classification Photosynthesis 01 natural sciences Chloroplast Thylakoids Diatom organelles 03 medical and health sciences 030104 developmental biology Diatom Biochemistry Algae [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Phaeodactylum tricornutum Plastid 010606 plant biology & botany |
| Zdroj: | Plastids: Methods and Protocols. Series: Methods in Molecular Biology Marechal Eric. Plastids: Methods and Protocols. Series: Methods in Molecular Biology, 1829, Springer, pp.189-203, 2018, Methods in Molecular Biology, 978-1-4939-8654-5; 978-1-4939-8653-8. ⟨10.1007/978-1-4939-8654-5_13⟩ Marechal Eric. Plastids: Methods and Protocols. Series: Methods in Molecular Biology, 1829, Springer, pp.189-203, 2018, ⟨10.1007/978-1-4939-8654-5_13⟩ Methods in Molecular Biology ISBN: 9781493986538 |
| DOI: | 10.1007/978-1-4939-8654-5_13⟩ |
| Popis: | International audience; The so-called "complex" plastids from diatoms possessing four envelope membranes are a typical feature of algae that arose from secondary endosymbiosis. Studying isolated plastids from these algae may allow answering a number of fundamental questions regarding diatom photosynthesis and plastid functionality. Due to their complex architecture and their integration into the cellular endoplasmic reticulum (ER) system, their isolation though is still challenging. In this work, we report a reliable isolation technique that is applicable for the two model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum. The resulting plastid-enriched fractions are of homogenous quality, almost free from cellular contaminants, and feature structurally intact thylakoids that are capable to perform oxygenic photosynthesis, though in most cases they seem to lack most of the stromal components as well as plastid envelopes. |
| Databáze: | OpenAIRE |
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