Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
| Autor: | Dušica Janošević, Snežana Budimir, Dragana Stojičić, Branka Uzelac, Svetlana Tošić, Bojan Zlatković, Violeta Slavkovska |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Plant Science Cyclohexane Monoterpenes Sesquiterpene 010603 evolutionary biology 01 natural sciences 7. Clean energy law.invention chemistry.chemical_compound Murashige and Skoog medium law Culture Techniques Botany Genetics Oils Volatile Essential oil Lamiaceae biology Adenine Trichomes Kinetin biology.organism_classification Menthone Menthol chemistry Micropropagation Microscopy Electron Scanning Monoterpenes Pulegone 010606 plant biology & botany |
| Zdroj: | Planta |
| Popis: | In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N6–benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 μM BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed. |
| Databáze: | OpenAIRE |
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