A novel programmed cell death protein 4 negatively regulates CgIL17-5 expression in hemocytes of oyster Pacific oyster (Crassostrea gigas)
| Autor: | Lingling Wang, Zhaoqun Liu, Weilin Wang, Meijia Li, Yunchen Yan, Linsheng Song, Wen Yang, Xiaojing Lv |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Oyster Hemocytes Aquatic Science Open Reading Frames 03 medical and health sciences Complementary DNA biology.animal Animals Environmental Chemistry Programmed Cell Death Protein 4 Crassostrea Peptide sequence Messenger RNA biology Gene Expression Profiling Interleukin-17 04 agricultural and veterinary sciences General Medicine Pacific oyster biology.organism_classification Immunity Innate Up-Regulation Cell biology Open reading frame 030104 developmental biology Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Fish & Shellfish Immunology. 99:594-602 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.02.038 |
| Popis: | The programmed cell death protein 4 (PDCD4) is a newly defined transcriptional and translational inhibitor, which plays a key role in regulating the synthesis of inflammatory cytokines in vertebrates species. In the present study, the full-length cDNA of PDCD4 from oyster Crassostrea gigas (designed as CgPDCD4) was identified to explore its possible involvement in immune response. The open reading frame of pdcd4 gene was of 1344 bp encoding a polypeptide of 447 amino acids with two conserved MA-3 domains. The deduced amino acid sequence of CgPDCD4 shared 60.18% similarity with PDCD4 from Mizuhopecten yessoensis. The mRNA transcripts of CgPDCD4 could be detected in all the tested tissues with a higher expression level in adductor muscle and hemocytes. The mRNA expression of CgPDCD4 in hemocytes was significantly down-regulated at 3 h and 6 h (0.61-fold and 0.42-fold of that in PBS group, p 0.01, respectively) after LPS stimulation. In hemocytes, CgPDCD4 protein was found to be mainly located in the cytoplasm. After the mRNA expression of CgPDCD4 in hemocytes was knocked down (0.40-fold of that in EGFP-RNAi group) by CgPDCD4 dsRNA (dsCgPDCD4) injection, the CgIL17-5 transcripts were up-regulated (20.11-fold of that in PBS group, p 0.01) post LPS stimulation, which was significantly higher than that in dsEGFP-injected oysters (7.06-fold of that in PBS group, p 0.01). Meanwhile, the nuclear translocation of CgRel (homologue of Rel/NF-κB) was significantly enhanced (about 1.36-fold of that in PBS group, p 0.01), but it was similar as that in EGFP-RNAi group (about 1.52-fold of that in PBS group, p 0.01) after LPS stimulation. All the results suggested that CgPDCD4 in oysters played the same role as PDCD4 of vertebrates in negatively regulating the production of interleukin in immune response, but the underpinning signal pathway was not conserved during evolution. |
| Databáze: | OpenAIRE |
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