Comparison of the protein content of three different bovine secretory granule membrane types: a search for exocytosis-specific shared proteins
| Autor: | R W Rubin, B C Pressman, A K Lyubkin |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Granule (cell biology)
Membrane Proteins Articles Cell Biology Biology Cell Fractionation Cytoplasmic Granules Secretory Vesicle Exocytosis Cell biology Microscopy Electron Membrane Membrane protein Animals Cattle Chromaffin Granules Electrophoresis Polyacrylamide Gel Integral membrane protein Secretory granule membrane Polyacrylamide gel electrophoresis |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.99.1.356 |
| Popis: | A two-dimensional polyacrylamide gel analysis of three types of bovine exocytotic granule membranes has been undertaken. Great care was taken to purify the membranes of biochemical homogeneity with minimal contamination from other membrane sources. The goal was to identify proteins that were present in all three membrane types. Although a number of minor components were observed that co-migrated for two membrane types, no proteins were detected that were present in all three granule membranes. We therefore conclude that such exocytosis-specific proteins do not exist or that they represent less than 0.1% of the total membrane protein present in a given isolated membrane preparation. |
| Databáze: | OpenAIRE |
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