FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum
| Autor: | Jinrong Yin, Xue Zhang, Guanghui Wang, Wei Wang, Jin-Rong Xu, Gang Niu, Chaofeng Hao, Ping Xiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Hypha
Cell division Mutant Morphogenesis Hyphae Biology Microbiology Fungal Proteins 03 medical and health sciences Fusarium Tip growth Ecology Evolution Behavior and Systematics Triticum 030304 developmental biology Plant Diseases 0303 health sciences Virulence 030306 microbiology fungi Fungal genetics Wild type Spores Fungal Cell biology Mutation Cytokinesis Cell Division |
| Zdroj: | Environmental microbiologyReferences. 22(12) |
| ISSN: | 1462-2920 |
| Popis: | Ascospores are the primary inoculum in Fusarium graminearum, a causal agent of wheat head blight. In a previous study, FgPAL1 was found to be upregulated in the Fgama1 mutant and important for ascosporogenesis. However, the biological function of this well-conserved gene in filamentous ascomycetes is not clear. In this study, we characterized its functions in growth, differentiation and pathogenesis. The Fgpal1 mutant had severe growth defects and often displayed abnormal hyphal tips. It was defective in infectious growth in rachis tissues and spreading in wheat heads. The Fgpal1 mutant produced conidia with fewer septa and more nuclei per compartment than the wild type. In actively growing hyphal tips, FgPal1-GFP mainly localized to the subapical collar and septa. The FgPal1 and LifeAct partially co-localized at the subapical region in an interdependent manner. The Fgpal1 mutant was normal in meiosis with eight nuclei in developing asci but most asci were aborted. Taken together, our results showed that FgPal1 plays a role in maintaining polarized tip growth and coordination between nuclear division and cytokinesis, and it is also important for infectious growth and developments of ascospores by the free cell formation process. |
| Databáze: | OpenAIRE |
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