In vitro and in vivo pharmacokinetics and toxicity evaluation of curcumin incorporated titanium dioxide nanoparticles for biomedical applications
| Autor: | S. Balachandran, Sathyabhama Sheeja, Rukhmini Sudha Devi, Rema Sreenivasan Soumya, Sainulabdeen Sherin, Annie Abraham |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Biodistribution Curcumin Erythrocytes Time Factors Cell Survival Metal Nanoparticles 02 engineering and technology Pharmacology Toxicology medicine.disease_cause Cell Line Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics medicine Animals Humans Tissue Distribution Viability assay Titanium Drug Carriers General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Rats Bioavailability Comet assay Drug Liberation Microscopy Fluorescence chemistry 030220 oncology & carcinogenesis Drug delivery Comet Assay 0210 nano-technology Genotoxicity Nuclear chemistry |
| Zdroj: | Chemico-Biological Interactions. 275:35-46 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/j.cbi.2017.07.022 |
| Popis: | The present study deals with the preparation of stable Curcumin incorporated Titaniumdioxide Nanoparticles (CTNPs) by coprecipitation method for improving the bioavailability of curcumin and site specific drug delivery. The prepared nanoparticles were characterized by UV visible spectroscopy, FTIR, XRD, DLS, SEM and EDX. The characterization studies showed the interaction of curcumin to titanium dioxide nanoparticles. The average size of the prepared CTNPs was found to be ∼29 nm with zetapotential of-53.790 mV. In vivo and in vitro toxicological evaluations were carried out to determine the biological effect of CTNPs. In vitro parameters like cell viability, Lactate dehydrogenase (LDH) Assay, Neutral red uptake (NRU) assay and uptake of curcumin from CTNPs by the cells had been investigated. In vitro toxicity studies in THP1 and H9c2 cell lines showed that CTNPs are safe even at a dose of 200 ng. The in vivo part of the study was carried out with different doses of Curcumin (1 mg–20 mg/kg body weight), Titaniumdioxide Nanoparticles (TNPs) (1 mg–5 mg/kg Body weight) and CTNPs (5 mg–10 mg/kg Body weight) in Sprague dawley rat models to determine the pharmacokinetics and genotoxicity of the nanoparticle. This was done by analysing the parameters like SGPT, SGOT, LDH, hematological parameters and biodistribution of the nanomaterial at different organ sites. Genotoxicity of samples were done by comet assay on blood cells. No significant toxicity was observed in the parameters in samples treated group compared to controls. The overall results indicated that the CTNPs are nontoxic and is highly stable with improved site specific application compared to native curcumin and are suitable for biomedical applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect