Spectroscopic studies of the aggregation behavior of Human Serum Albumin and cetyltrimethylammonium bromide
Autor: | Rachana Srivastava, Md. Sayem Alam |
---|---|
Rok vydání: | 2020 |
Předmět: |
Circular dichroism
macromolecular substances 02 engineering and technology Biochemistry Micelle Hydrophobic effect 03 medical and health sciences chemistry.chemical_compound Pulmonary surfactant Structural Biology Bromide medicine Molecular Biology 030304 developmental biology 0303 health sciences technology industry and agriculture General Medicine 021001 nanoscience & nanotechnology Human serum albumin Fluorescence body regions Crystallography chemistry Critical micelle concentration 0210 nano-technology medicine.drug |
Zdroj: | International Journal of Biological Macromolecules. 158:394-400 |
ISSN: | 0141-8130 |
Popis: | To check the role of micelle in the interaction studies of human serum albumin (HSA) and cetyltrimethylammonium bromide (CTAB), many spectroscopic techniques, like UV–visible, fluorescence, circular dichroism, fluorescence lifetime measurement, and atomic force microscopy (AFM), are employed. The binding affinity of all compound groups depended on the hydrocarbon chain, indicating the predominant role of hydrophobic forces, electrostatic forces and supported by polar interactions on protein surfaces. The protein has a different effect on the polarity of a microenvironment in fluorescence spectra above and below the critical micelle concentration (CMC) of the suractant. The far-UV-CD spectra show unfolding below the CMC and refolding above the CMC. The binding of the surfactant induces changes in the microenvironment at different pHs around the residues of the aromatic amino acid and the disulfide bond of protein. The AFM images show significant changes in the protein's structure. AFM images show dense aggregation below the CMC and above the CMC, some net-like structure formed in the HSA-CTAB complex. To test the experimental results, we used Auto dock Vina to conduct molecular docking. Above and below the CMC, structural changes can be seen. |
Databáze: | OpenAIRE |
Externí odkaz: |