Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families

Autor: Genevieve Renauld, Camille Locht, M C Geoffroy
Přispěvatelé: Génomes, biologie cellulaire et thérapeutiques (GenCellDi (UMR_S_944)), Collège de France (CdF (institution))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)
Rok vydání: 1992
Předmět:
DNA
Bacterial
Chaperonins
Operon
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Biological Transport
Active
Sequence alignment
General Biochemistry
Genetics and Molecular Biology
Bordetella pertussis
Microbiology
03 medical and health sciences
Gene interaction
Bacterial Proteins
Sequence Homology
Nucleic Acid
Consensus Sequence
Consensus sequence
Amino Acid Sequence
RNA
Messenger
Virulence Factors
Bordetella
Adhesins
Bacterial
Molecular Biology
Peptide sequence
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Genetics
0303 health sciences
Adhesins
Escherichia coli
General Immunology and Microbiology
biology
Base Sequence
Virulence
030306 microbiology
General Neuroscience
Structural gene
Chromosome Mapping
Proteins
biology.organism_classification
Bordetella
Mutagenesis
Insertional
Hemagglutinins
Pilin
Fimbriae
Bacterial
biology.protein
bacteria
Bacterial Outer Membrane Proteins
Flagellin
Research Article
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 1992, 11 (9), pp.3175-3183. ⟨10.1002/j.1460-2075.1992.tb05394.x⟩
ISSN: 0261-4189
1460-2075
DOI: 10.1002/j.1460-2075.1992.tb05394.x⟩
Popis: The Bordetella pertussis filamentous hemagglutinin (FHA) is a major virulence factor responsible for attachment, one of the early events in bacterial pathogenesis. Deletion of its structural gene, fhaB, or a Tn5 insertion in fhaA, downstream of fhaB, resulted in a FHA- and fimbriae- phenotype, although fhaB and the fim genes are not linked. The fhaB downstream region therefore most likely encodes accessory proteins required for the biosynthesis of FHA and fimbriae, despite the lack of sequence similarities between these two proteins. The nucleotide sequence of this area contains the open reading frames fhaD and fhaA, whose products share sequence similarities with the papD and papC gene products, respectively. PapD is a periplasmic chaperone protein able to bind to the Escherichia coli P pilin subunits and to transport them towards the outer membrane protein PapC which is responsible for pilus membrane translocation. An additional open reading frame, fhaE, is located downstream of fhaA. Its amino acid sequence shares similarities with those of the fimbrial subunits. Deletion analyses suggest that fhaB and the downstream genes can be transcribed as a polycistronic operon, and primer extension analysis revealed the presence of a second promoter between fhaB and fhaD.
Databáze: OpenAIRE
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