Intermediate-conductance Ca2+-activated K+ channels (IKCa1) regulate human prostate cancer cell proliferation through a close control of calcium entry
| Autor: | Brigitte Mauroy, Jean-Louis Bonnal, Florian Gackière, Pascal Mariot, Philippe Delcourt, Pierre Gosset, Hélène Lallet-Daher, R Urbain, Christian Slomianny, Natalia Prevarskaya, Etienne Dewailly, Roman Skryma, Alexis Bavencoffe, L Fleurisse, Morad Roudbaraki, Gabriel Bidaux |
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| Přispěvatelé: | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Institut National de la Sante et de la Recherche Medicale (Inserm)European Commission Ministere de l'Education Nationale de l'Enseignement Supe'rieur et de la Recherche Ligue nationale contre le cancer Association pour la Recherche sur les Tumeurs de la Prostate (ARTP) |
| Rok vydání: | 2009 |
| Předmět: |
Male
Cancer Research [SDV]Life Sciences [q-bio] intermediate-conductance potassium channels Membrane Potentials Transient receptor potential channel 0302 clinical medicine Membrane potential 0303 health sciences S100 Proteins Intracellular Signaling Peptides and Proteins prostate cancer Intermediate-Conductance Calcium-Activated Potassium Channels Potassium channel 3. Good health Cell biology 030220 oncology & carcinogenesis capacitative calcium entry Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinase Inhibitor p21 medicine.medical_specialty TRPV6 proliferation chemistry.chemical_element TRPV Cation Channels Calcium Biology patch clamp 03 medical and health sciences Calcium imaging Internal medicine Cell Line Tumor Genetics medicine Humans RNA Messenger Molecular Biology 030304 developmental biology Cell Proliferation Cell growth T-type calcium channel G1 Phase Prostatic Neoplasms Endocrinology chemistry Benzimidazoles Calcium Channels Tumor Suppressor Protein p53 |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2009, 28 (15), pp.1792-1806. ⟨10.1038/onc.2009.25⟩ |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/onc.2009.25⟩ |
| Popis: | International audience; Accumulating data point to K þ channels as relevant players in controlling cell cycle progression and proliferation of human cancer cells, including prostate cancer (PCa) cells. However, the mechanism(s) by which K þ channels control PCa cell proliferation remain illusive. In this study, using the techniques of molecular biology, biochemistry, electrophysiology and calcium imaging, we studied the expression and functionality of intermediate-conductance calcium-activated potassium channels (IK Ca1) in human PCa as well as their involvement in cell proliferation. We showed that IK Ca1 mRNA and protein were preferentially expressed in human PCa tissues, and inhibition of the IK Ca1 potassium channel suppressed PCa cell proliferation. The activation of IK Ca1 hyperpolarizes membrane potential and, by promoting the driving force for calcium, induces calcium entry through TRPV6, a cation channel of the TRP (Transient Receptor Potential) family. Thus, the overexpression of the IK Ca1 channel is likely to promote carcinogenesis in human prostate tissue. |
| Databáze: | OpenAIRE |
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