The blood lactate/pyruvate equilibrium affair
Autor: | Jose A. Arevalo, George A. Brooks, Robert G. Leija, Adam D. Osmond, Justin Duong, Casey C. Curl, Michael A. Horning |
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Rok vydání: | 2022 |
Předmět: |
medicine.medical_specialty
Magnetic Resonance Spectroscopy isotope tracers Physiology Endocrinology Diabetes and Metabolism Rest Mass spectrometry Medical and Health Sciences Mass Spectrometry Endocrinology & Metabolism Dogs Physiology (medical) Internal medicine oxidative metabolism Pyruvic Acid Blood lactate medicine Animals Humans Glycolysis Carbon Radioisotopes Lactic Acid Radioactive Tracers Muscle Skeletal Exercise Lung energy-substrate partitioning Chemistry Metabolism Skeletal Femoral Vein glycolysis Biological Sciences Immunohistochemistry Femoral Artery Kinetics Endocrinology Biochemistry Muscle Lactate shuttle hypothesis Perspectives Signal Transduction |
Zdroj: | American journal of physiology. Endocrinology and metabolism, vol 322, iss 1 Am J Physiol Endocrinol Metab |
Popis: | The Lactate Shuttle hypothesis is supported by a variety of techniques including mass spectrometry analytics following infusion of carbon-labeled isotopic tracers. However, there has been controversy over whether lactate tracers measure lactate (L) or pyruvate (P) turnover. Here, we review the analytical errors, use of inappropriate tissue and animal models, failure to consider L and P pool sizes in modeling results, inappropriate tracer and blood sampling sites, and failure to anticipate roles of heart and lung parenchyma on L⇔P interactions. With support from magnetic resonance spectroscopy (MRS) and immunocytochemistry, we conclude that carbon-labeled lactate tracers can be used to quantitate lactate fluxes. |
Databáze: | OpenAIRE |
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