The blood lactate/pyruvate equilibrium affair

Autor: Jose A. Arevalo, George A. Brooks, Robert G. Leija, Adam D. Osmond, Justin Duong, Casey C. Curl, Michael A. Horning
Rok vydání: 2022
Předmět:
Zdroj: American journal of physiology. Endocrinology and metabolism, vol 322, iss 1
Am J Physiol Endocrinol Metab
Popis: The Lactate Shuttle hypothesis is supported by a variety of techniques including mass spectrometry analytics following infusion of carbon-labeled isotopic tracers. However, there has been controversy over whether lactate tracers measure lactate (L) or pyruvate (P) turnover. Here, we review the analytical errors, use of inappropriate tissue and animal models, failure to consider L and P pool sizes in modeling results, inappropriate tracer and blood sampling sites, and failure to anticipate roles of heart and lung parenchyma on L⇔P interactions. With support from magnetic resonance spectroscopy (MRS) and immunocytochemistry, we conclude that carbon-labeled lactate tracers can be used to quantitate lactate fluxes.
Databáze: OpenAIRE