Multidrug Resistance Associated Protein 1 protects against bilirubin-induced cytotoxicity
| Autor: | Graciela Luján Mazzone, Leslye Roca-Burgos, J. Donald Ostrow, Dean Cekic, Fabio Gerin, Claudio Tiribelli, Sebastian Calligaris |
|---|---|
| Přispěvatelé: | Calligaris, S, Cekic, D, ROCA BURGOS, L, Gerin, F, Mazzone, G, Ostrow, Jd, Tiribelli, Claudio |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Bilirubin
Cell Survival Biophysics Biology Tritium Biochemistry chemistry.chemical_compound Mice Adenosine Triphosphate Structural Biology Lactate dehydrogenase Genetics Animals Cytotoxicity Molecular Biology Cells Cultured Mice Knockout Dose-Response Relationship Drug Wild type Cell Biology Fibroblasts Molecular biology chemistry embryonic structures Toxicity Multidrug Resistance-Associated Protein 1 Multidrug Resistance-Associated Proteins Adenosine triphosphate Intracellular |
| Popis: | We have shown that multidrug resistance associated protein 1 (MRP1) mediates ATP-dependent extrusion of bilirubin, possibly limiting its potentially toxic accumulation in cells. To determine directly if Mrp1 protects cells against unconjugated bilirubin (UCB) toxicity, mouse embryo fibroblasts (MEF) were isolated from Mrp1 knockout (−/−) mice and their wild type (WT) (+/+) littermates. Compared to WT cells, cultured MEF (−/−) cells exposed to 40–140nM unbound [H3]-bilirubin accumulated twice as much [H3]-bilirubin (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|