Comparison of slurry mixing and dry milling in laboratory sample preparation for determination of ochratoxin A and deoxynivalenol in wheat
| Autor: | Vincenzo Lippolis, Stefania Valenzano, Angelo Visconti, Michelangelo Pascale |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pharmacology
Ochratoxin A Accuracy and precision Chromatography High variability Mixing (process engineering) Food Contamination Repeatability Ochratoxins Chemistry Techniques Analytical Analytical Chemistry chemistry.chemical_compound chemistry Carcinogens Slurry Environmental Chemistry Sample preparation Positive skewness Trichothecenes Agronomy and Crop Science Food Analysis Triticum Food Science Mathematics |
| Zdroj: | Journal of AOAC International 95 (2012): 452–458. doi:10.5740/jaoacint.11-047 info:cnr-pdr/source/autori:Lippolis V., Pascale M., Valenzano S., Visconti A/titolo:Comparison of slurry mixing and dry milling in laboratory sample preparation for determination of ochratoxin A and deoxynivalenol in wheat/doi:10.5740%2Fjaoacint.11-047/rivista:Journal of AOAC International/anno:2012/pagina_da:452/pagina_a:458/intervallo_pagine:452–458/volume:95 |
| DOI: | 10.5740/jaoacint.11-047 |
| Popis: | The significance of laboratory sample preparation for the determination of two important mycotoxins, ochratoxin A (OTA) and deoxynivalenol (DON), in wheat was investigated by comparing water-slurry mixing and dry-milling procedures. The distribution of OTA and DON in 10 kg samples of naturally contaminated wheat was established by analyzing one hundred 100 g subsamples of each sample. A normal distribution and a good repeatability of DON measurements was observed for both water-slurry mixing (mean 2290 μg/kg, CV 4.6%, median 2290 μg/kg) and dry milling (mean 2310 μg/kg, CV 6.4%, median 2290 μg/kg) procedures. For OTA determinations, reliable results could be obtained only by slurry mixing sample preparation (mean 2.62 μg/kg, CV 4.0%, median 2.62 μg/kg), whereas dry-milling comminution resulted in an inhomogeneous distribution with a high variability (mean 0.83 μg/kg, CV 75.2%, median 0.60 μg/kg) and a positive skewness (2.12). Ad hoc experiments were performed on different size portions of the same sample (10 kg) to assess accuracy and precision of the comminution/homogenization procedures (slurry mixing and dry milling). Very good results were obtained for DON determination with both procedures in terms of accuracy (>98.7% of the “weighted value”) and precision (CV |
| Databáze: | OpenAIRE |
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