Formulation and Storage of Platelet-Rich Plasma Homemade Product
| Autor: | Florence Sabatier, Christophe Dubois, Olivier Bausset, Guy Magalon, Nicolas Serratrice, Laurent Giraudo, Jean-Marie Coudreuse, Françoise Dignat-George, Jeremy Magalon, Julie Veran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Chromatography medicine.diagnostic_test business.industry Growth factor medicine.medical_treatment lcsh:R lcsh:Medicine General Biochemistry Genetics and Molecular Biology Thromboelastography Article Flow cytometry Adenosine diphosphate chemistry.chemical_compound chemistry lcsh:Biology (General) Platelet-rich plasma medicine Centrifugation Platelet Platelet activation business lcsh:QH301-705.5 |
| Zdroj: | BioResearch Open Access, Vol 1, Iss 3, Pp 115-123 (2012) BioResearch Open Access |
| ISSN: | 2164-7860 |
| DOI: | 10.1089/BIORES.2012.0225 |
| Popis: | The platelet-rich plasma (PRP) is an autologous biotherapy based on platelet-healing properties. Here, we developed a simple and reproducible PRP purification protocol based on two successive centrifugations. We evaluated different centrifugation speeds and time-storage durations on the platelet quantity and quality. Sterility and stability of our PRP homemade product were also performed. We prepared PRP from 54 healthy volunteers. We tested activation state, reactivity, and stability of platelets by flow cytometry using basal and adenosine diphosphate (ADP)-induced P-selectin expression markers; growth factor release after platelet activation by an enzyme-linked immunosorbent assay (ELISA); platelet aggregation capacity by aggregrometry assays; clot formation and retraction by thromboelastography; and platelet morphology by ultrastructural analysis. About 130 and 250 g successive speed centrifugations further concentrated platelets while preserving their bioactivity during 6 h (after that, platelet functions were significantly altered). In these conditions, we obtained a highly concentrated pure PRP product (with a low leukocyte count) suitable to study platelet properties. To avoid the loss of efficacy, we recommend injecting PRP under 3 h after preparation. |
| Databáze: | OpenAIRE |
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