Enhancement of cell-mediated immunity against HIV-1 induced by coinnoculation of plasmid-encoded HIV-1 antigen with plasmid expressing IL-12
| Autor: | Tsuji, T., Hamajima, K., Fukushima, J., Xin, Kq, Ishii, N., Aoki, I., Ishigatsubo, Y., Tani, K., Kawamoto, S., Nitta, Y., Jun-ichi Miyazaki, Koff, Wc, Okubo, T., Okuda, K. |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | ResearcherID |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.158.8.4008 |
| Popis: | Previous investigations have demonstrated that CTL may play an important role in suppressing the disease progression of HIV infection. In this study, we inoculated mice with IL-12 expression plasmid together with plasmid-encoding HIV-1 envelope to enhance CTL activity by activating a Th1-type response. The results of delayed-type hypersensitivity using the footpad swelling response and of CTL activity clearly showed that HIV-1-specific cell-mediated immunity was enhanced by inoculation of the IL-12 expression plasmid. Quantitation of cytokine in the sera of IL-12-inoculated mice revealed that IFN-gamma significantly increased. The enhanced cell-mediated immunity responses were abrogated by combined administration of the IL-12 expression plasmid and neutralizing anti-IFN-gamma Ab. Together, these results suggest that enhanced virus-specific cell-mediated immunity occurred via an endogenously produced IFN-gamma by inoculation of IL-12 expression plasmid. |
| Databáze: | OpenAIRE |
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