Nasal immunization of mice with Lactobacillus casei expressing the Pneumococcal Surface Protein A: induction of antibodies, complement deposition and partial protection against Streptococcus pneumoniae challenge
| Autor: | Débora A. Silva, Maria Leonor S. Oliveira, Michelle Darrieux, Ivana B. Campos, Karina Araújo Aires, Luciana C. C. Leite, Ana Paula Mattos Arêas, E. N. Miyaji, Daniela M. Ferreira, Paulo Lee Ho |
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| Rok vydání: | 2007 |
| Předmět: |
Serotype
Lactobacillus casei Immunology Genetic Vectors medicine.disease_cause Microbiology Pneumococcal Infections Pneumococcal Vaccines Mice Immune system Antigen Bacterial Proteins Lactobacillus Streptococcus pneumoniae medicine Animals Complement Activation Immunity Mucosal Administration Intranasal Antigens Bacterial biology biology.organism_classification Virology Antibodies Bacterial Mice Inbred C57BL Lacticaseibacillus casei Infectious Diseases Immunization Immunoglobulin G biology.protein Female Transformation Bacterial Antibody Plasmids |
| Zdroj: | Microbes and infection. 10(5) |
| ISSN: | 1286-4579 |
| Popis: | Strategies for the development of new vaccines against Streptococcus pneumoniae infections try to overcome problems such as serotype coverage and high costs, present in currently available vaccines. Formulations based on protein candidates that can induce protection in animal models have been pointed as good alternatives. Among them, the Pneumococcal Surface Protein A (PspA) plays an important role during systemic infection at least in part through the inhibition of complement deposition on the pneumococcal surface, a mechanism of evasion from the immune system. Antigen delivery systems based on live recombinant lactic acid bacteria (LAB) represents a promising strategy for mucosal vaccination, since they are generally regarded as safe bacteria able to elicit both systemic and mucosal immune responses. In this work, the N-terminal region of clade 1 PspA was constitutively expressed in Lactobacillus casei and the recombinant bacteria was tested as a mucosal vaccine in mice. Nasal immunization with L. casei-PspA 1 induced anti-PspA antibodies that were able to bind to pneumococcal strains carrying both clade 1 and clade 2 PspAs and to induce complement deposition on the surface of the bacteria. In addition, an increase in survival of immunized mice after a systemic challenge with a virulent pneumococcal strain was observed. |
| Databáze: | OpenAIRE |
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