Isolation and Partial Characterization of Lactic Acid Bacteria from the Gut Microbiota of Marine Fishes for Potential Application as Probiotics in Aquaculture
| Autor: | Inés García de la Banda, M. Carmen Castro, Xabier Moreno-Ventas, Sergio Alonso, Margarita Berdasco, Alma Hernández de Rojas |
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| Přispěvatelé: | Universidad de Cantabria |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Marine Biology Aquaculture Gut flora Microbiology Bile Acids and Salts 03 medical and health sciences Anti-Infective Agents Lactobacillales Animals Food science Molecular Biology biology Vibrio harveyi Probiotics Lactococcus lactis Fishes Hydrogen-Ion Concentration biology.organism_classification Antimicrobial Gastrointestinal Microbiome 030104 developmental biology Photobacterium damselae Leuconostoc mesenteroides Molecular Medicine Bacteria Lactobacillus plantarum |
| Zdroj: | Probiotics and Antimicrobial Proteins 29 June pp 1-11 UCrea Repositorio Abierto de la Universidad de Cantabria Universidad de Cantabria (UC) |
| Popis: | With the increase of antimicrobial resistances due to the widespread use of antibiotics, the search of new probiotics to control aquaculture diseases has a growing public interest. The aim of this study was to isolate bacteria with antimicrobial effect from the gut of marine healthy fishes and select lactic acid bacteria (LAB) as potential probiotics, being strains considered as generally regarded as safe (GRAS) by the European Food Safety Agency (EFSA). Of a total of 45 Gram-positive strains with antimicrobial activity found in a screening of the gut microbiota of 13 marine fishes, nine were identified as LAB by 16S rRNA gene sequencing. LAB strains (five Lactococcus lactis subsp. lactis, two Enterococcus spp., one Lactobacillus plantarum, and one Leuconostoc mesenteroides subsp. mesenteroides) also showed a broad-spectrum antibacterial activity against aquaculture pathogens such as Vibrio harveyi, V. splendidus, and Photobacterium damselae and survived in experimental gastrointestinal conditions when grown in culture media modified with different values of pH and bile salts. These results showed the potential of LAB obtained from the indigenous microbiota of wild marine fishes for use as probiotics in aquaculture. |
| Databáze: | OpenAIRE |
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