Immunogenicity of the recombinant serine rich Entamoeba histolytica protein (SREHP) amebiasis vaccine in the African Green Monkey
| Autor: | Tonghai Zhang, Kairong Tian, James Blanchard, Nakiisa Johnson, Frank B. Cogswell, Samuel L. Stanley, Lynne Foster, Cindy Kunz-Jenkins |
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| Rok vydání: | 1995 |
| Předmět: |
Protozoan Vaccines
Recombinant Fusion Proteins Protozoan Proteins Antibodies Protozoan Biology Microbiology law.invention Maltose-binding protein Entamoeba histolytica law Chlorocebus aethiops Serine Animals Antiserum Vaccines Synthetic Entamoebiasis General Veterinary General Immunology and Microbiology Immunogenicity Public Health Environmental and Occupational Health Membrane Proteins Vaccine efficacy biology.organism_classification Virology Vaccination Disease Models Animal Infectious Diseases Liver Abscess Amebic biology.protein Recombinant DNA Molecular Medicine Antibody |
| Zdroj: | Vaccine. 13:947-951 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/0264-410x(95)00001-h |
| Popis: | We report the first study in non-human primates of the safety and immunogenicity of a recombinant vaccine designed to prevent amebic liver abscess. In a pilot study, a recombinant vaccine containing the serine rich Entamoeba histolytica protein (SREHP) attached to a maltose binding protein (SREHP/MBP), which has been shown to be effective in preventing amebic liver abscess in rodent models of infection, was used to immunize two African Green Monkeys. Vaccination with SREHP/MBP resulted in no systemic side-effects. The monkeys receiving the SREHP/MBP protein developed antibodies that recognized the recombinant SREHP/MBP molecule, the native SREHP protein, and the surface of amebic trophozoites. Antiserum from SREHP/MBP-vaccinated monkeys could block the adhesion of E. histolytica trophozoites to mammalian cells, a feature that may correlate with vaccine efficacy. Attempts to produce amebic liver abscess in naive African Green Monkeys by direct hepatic inoculation with virulent E. histolytica trophozoites was not successful, suggesting this species is probably not suitable for vaccine efficacy studies. |
| Databáze: | OpenAIRE |
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