ANTIGENICITY TESTS OF SUPLATAST TOSILATE (IPD-1151T)
| Autor: | Akinobu Ohuchida, Shigenori Katayama, Yasuhiro Maeda, Mitsuo Kurokawa, Shigeo Sugimoto, Hiroshi Manabe, Yasuhide Kouchi, Ryouichi Yoshida, Takaaki Mizumoto, Masahiro Nakagawa |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Antigenicity Salmonella Injections Subcutaneous Guinea Pigs Histamine Antagonists Sulfonium Compounds Administration Oral Toxicology medicine.disease_cause Chinese hamster Mice In vivo medicine Animals Arylsulfonates Anaphylaxis Escherichia coli Mice Inbred BALB C Mice Inbred C3H biology Rats Inbred Strains bacterial infections and mycoses biology.organism_classification Molecular biology In vitro Rats Suplatast tosilate Antibody Formation Micronucleus test Immunology Injections Intraperitoneal |
| Zdroj: | The Journal of Toxicological Sciences. 17:221-233 |
| ISSN: | 1880-3989 0388-1350 |
| DOI: | 10.2131/jts.17.supplementii_221 |
| Popis: | 1. The reverse mutation test was carried out on suplatast tosilate (IPD-1151T) at dose range of 50-5000 micrograms/plate using Salmonella typhimurium strains TA100, TA1535, TA98 TA1538 and TA1537, and Escherichia coli strains WP2, WP2uvrA. In all tester strains no significant differences were observed in the number of revertant colonies as compared with solvent control in the absence or presence of mammalian metabolic activation system. 2. The chromosomal aberration test on IPD-1151T was carried out using cultured Chinese hamster lung cells (CHL). The cells were treated with IPD-1151T at the doses of 125, 250 and 500 micrograms/ml without S9 Mix and at the doses of 1250, 2500 and 5000 micrograms/ml with S9 Mix. The incidence of structural- and numeral-aberration was 0-4% in the absence or presence of mammalian metabolic activation system, no significant increases were observed in the incidence of chromosomal aberrations. 3. The micronucleus test using BDF1 male mice was conducted in order to evaluate the in vivo mutagenicity of IPD-1151T. IPD-1151T was orally administered at doses of 625, 1250, 2500 and 5000 mg/kg, with a sampling time of 24 hr. The frequency of polychromatic erythrocytes with micronuclei (MNPCE) was 0.23% in the lowest dose (625mg/kg) of IPD-1151T, but the frequency of MNPCE was 0.03-0.13% in the groups treated with 1250-5000 mg/kg of IPD-1151T and no significant increases were observed with dose dependence. The results indicated that IPD-1151T was negative, even in the assessment standard using our background data. 4. The present study indicates that IPD-1151T has no in vitro and in vivo mutagenic potential. |
| Databáze: | OpenAIRE |
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