Estradiol-17β Upregulates Pyruvate Kinase M2 Expression to Coactivate Estrogen Receptor-α and to Integrate Metabolic Reprogramming With the Mitogenic Response in Endometrial Cells
| Autor: | Marwa Kamel, Gokhan S. Kilic, Concepcion R. Diaz-Arrastia, Mahmoud A. Mohammad, Salama A. Salama, Bih T. Ndofor, Mohamed S. AbdelBaki, Shaleen K. Theiler |
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| Rok vydání: | 2014 |
| Předmět: |
Stromal cell
Transcription Genetic Endocrinology Diabetes and Metabolism Primary Cell Culture Pyruvate Kinase Clinical Biochemistry Carbohydrate metabolism PKM2 Biology Biochemistry Endometrium Oxygen Consumption Endocrinology Humans Glycolysis Lactic Acid Cell Proliferation Estradiol Biochemistry (medical) Estrogen Receptor alpha Estrogens Cellular Reprogramming Cell biology Alternative Splicing Glucose Female Mitogens Stromal Cells Signal transduction Reprogramming Estrogen receptor alpha Pyruvate kinase Signal Transduction |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 99:3790-3799 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jc.2013-2639 |
| Popis: | Proliferating cells reprogram their cellular glucose metabolism to meet the bioenergetic and biosynthetic demands and to maintain cellular redox homeostasis. Pyruvate kinase M (PKM) is a critical regulator of this metabolic reprogramming. However, whether estradiol-17β (E2) reprograms cellular metabolism to support proliferation of human primary endometrial stromal cells (hESCs) and the molecular basis of this reprogramming are not well understood.Our objectives were to study whether E2 induces reprogramming of glucose metabolism in hESCs and to investigate the potential roles of PKM2 in E2-induced metabolic reprogramming and proliferation of these cells.The oxygen consumption rate and extracellular acidification rate were assessed by a Seahorse XF24 analyzer. PKM2 expression was assessed by real-time RT-PCR and immunoblotting.E2 induces a Warburg-like glucose metabolism in hESCs by inducing the expression of PKM. E2 also enhanced PKM splicing into the PKM2 isoform by upregulating the c-Myc-hnRNP axis. Furthermore, E2 induces PKM2 oxidation, phosphorylation, and nuclear translocation. In addition to its glycolytic function, PKM2 physically interacted with estrogen receptor-α (ERα) and functioned as an ERα coactivator. Small-molecule PKM2 activators ameliorated ERα transcriptional activity and abrogated the E2-induced hESC proliferation.We show for the first time that E2-induced hESC proliferation is associated with a shift in glucose metabolism toward aerobic glycolysis, and the molecular basis for this metabolic shift is linked to the effects of E2 on PKM2. In addition, PKM2 acts as a transcriptional coactivator for ERα and small-molecule PKM2 activators inhibit ERα transcriptional activity and reduce E2-induced cell proliferation. |
| Databáze: | OpenAIRE |
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