Human Papilloma Virus (HPV)-E6/E7 and Epidermal Growth Factor Receptor (EGF-R) Protein Levels in Cervical Cancer and Cervical Intraepithelial Neoplasia (CIN)
| Autor: | Roger C. Young, Philip F. Rust, Subbi Mathur, Rajesh S. Mathur |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Papillomavirus E7 Proteins Immunology Uterine Cervical Neoplasms Cervix Uteri Cervical intraepithelial neoplasia Basal (phylogenetics) Epidermal growth factor medicine Humans Immunology and Allergy Epidermal growth factor receptor Papillomaviridae Fluorescent Antibody Technique Indirect Cervical cancer biology virus diseases Obstetrics and Gynecology Epithelial Cells Oncogene Proteins Viral Uterine Cervical Dysplasia medicine.disease biology.organism_classification female genital diseases and pregnancy complications DNA-Binding Proteins ErbB Receptors Repressor Proteins Reproductive Medicine Carcinoma Squamous Cell biology.protein Cancer research Female Antibody |
| Zdroj: | American Journal of Reproductive Immunology. 46:280-287 |
| ISSN: | 1600-0897 8755-8920 |
| DOI: | 10.1034/j.1600-0897.2001.d01-14.x |
| Popis: | BACKGROUND: About 90% of cervical cancers and advanced cervical intraepithelial neoplasia (CIN II/III) are squamous epithelial cells with mRNA for human papillomavirus (HPV)16 and 18 and up-regulated epidermal growth factor receptor (EGF-R). Since presence of proteins rather than mRNA may be truly indicative of active infection or disease progression, establishing reliable methods for quantifying these proteins in cervical biopsies is important. METHOD: We have established an objective semi-quantitative immunofluorescent antibody assay to reliably assess the levels of HPV-E6/E7 and EGF-R proteins in the cervical biopsies from 12 normal women, five women with CIN I, 15 with CIN II/III and ten with cervical cancer. RESULTS: HPV-E6/E7 and EGF-R, when present, were specific to para-basal, basal and squamous epithelial cells (negative in stromal cells). Nine of ten women with cervical cancer and 15 (14 CIN II/III; 1 CIN I) of 20 women with CIN were positive for HPV-E6/E7. All 12 controls were HPV-negative. The controls and six women with CIN (four with CIN I) negative for HPV had low levels of EGF-R. The only exception was one woman with cervical cancer negative for HPV, with high levels of EGF-R. Levels of HPV-E6/E7 and EGF-R were significantly higher (P |
| Databáze: | OpenAIRE |
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