Sex differences in SR Ca2+ release in murine ventricular myocytes are regulated by the cAMP/PKA pathway

Autor: Laura A. Bienvenu, Randi J. Parks, Susan E. Howlett, Gibanananda Ray, Robert A. Rose
Rok vydání: 2014
Předmět:
Male
Intracellular Space
030204 cardiovascular system & hematology
Ryanodine receptor 2
chemistry.chemical_compound
0302 clinical medicine
Diastole
Calcium transient
Cyclic AMP
Myocyte
Myocytes
Cardiac
Phosphorylation
Gain
Excitation Contraction Coupling
Sex Characteristics
Calcium spark
0303 health sciences
Forskolin
Chemistry
Phosphodiesterase
Sarcoplasmic Reticulum
Female
Cardiology and Cardiovascular Medicine
Ion Channel Gating
Adenylyl Cyclases
Signal Transduction
medicine.medical_specialty
G protein
Heart Ventricles
Phosphodiesterase 3
Excitation–contraction coupling
03 medical and health sciences
Internal medicine
medicine
Animals
Cyclic adenosine monophosphate
Calcium Signaling
RNA
Messenger
Protein kinase A
Molecular Biology
030304 developmental biology
Gender
Ryanodine Receptor Calcium Release Channel
Cyclic AMP-Dependent Protein Kinases
Cyclic Nucleotide Phosphodiesterases
Type 4
Enzyme Activation
Mice
Inbred C57BL
Endocrinology
Calcium
Zdroj: Journal of Molecular and Cellular Cardiology. 75:162-173
ISSN: 0022-2828
DOI: 10.1016/j.yjmcc.2014.07.006
Popis: Previous studies have shown that ventricular myocytes from female rats have smaller contractions and Ca(2+) transients than males. As cardiac contraction is regulated by the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, we hypothesized that sex differences in cAMP contribute to differences in Ca(2+) handling. Ca(2+) transients (fura-2) and ionic currents were measured simultaneously (37�C, 2Hz) in ventricular myocytes from adult male and female C57BL/6 mice. Under basal conditions, diastolic Ca(2+), sarcoplasmic reticulum (SR) Ca(2+) stores, and L-type Ca(2+) current did not differ between the sexes. However, female myocytes had smaller Ca(2+) transients (26% smaller), Ca(2+) sparks (6% smaller), and excitation-contraction coupling gain in comparison to males (23% smaller). Interestingly, basal levels of intracellular cAMP were lower in female myocytes (0.7�0.1 vs. 1.7�0.2fmol/?g protein; p
Databáze: OpenAIRE
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