High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids
| Autor: | Stefan Dobosz, Konrad Ocalewicz, Tomasz Zalewski, Krzysztof Jagiełło, Oliwia Michalik |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system animal structures Article Subject Hydrostatic pressure lcsh:Medicine Zoology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Brown trout Homologous chromosome Salmo Larva Zygote General Immunology and Microbiology biology urogenital system lcsh:R 04 agricultural and veterinary sciences General Medicine Anatomy biology.organism_classification 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Rainbow trout Ploidy |
| Zdroj: | BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2017/2975187 |
| Popis: | Mitotic gynogenesis results in the production of fully homozygous individuals in a single generation. Since inbred fish were found to exhibit an increased frequency of body deformations that may affect their survival, the main focus of this research was to evaluate the ratio of individuals with spinal deformities among gynogenetic doubled haploids (DHs) brown trout as compared to nonmanipulated heterozygous individuals. Gynogenetic development was induced by the activation of brown trout eggs by UV-irradiated homologous and heterologous (rainbow trout) spermatozoa. The subsequent exposure of the activated eggs to the high hydrostatic pressure disturbed the first cleavage in gynogenetic zygotes and enabled duplication of the maternal haploid set of chromosomes. The survival rate was significantly higher among gynogenetic brown trout hatched from eggs activated with the homologous UV-irradiated spermatozoa when compared to DHs hatched from eggs activated by the heterologous spermatozoa. More than 35% of the gynogenetic larvae exhibited body deformities, mostly lordosis and scoliosis. The percentage of malformed brown trout from the control group did not exceed 15%. The increased number of deformed larvae among DHs brown trout suggested rather a genetic background of the disease related to the fish spine deformities; however, both genetic and environmental factors were discussed as a cause of such conditions in fish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|