Role of the Nuclease Activities Encoded by Herpes Simplex Virus 1 UL12 in Viral Replication and Neurovirulence
| Autor: | Michio Mugitani, Akihisa Kato, Zhuoming Liu, Shumpei Tsuda, Jun Arii, Yasushi Kawaguchi, Naoto Koyanagi, Hikaru Fujii |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Viral pathogenesis
viruses Immunology DNA Recombinant Herpesvirus 1 Human Biology medicine.disease_cause Virus Replication Microbiology Single-stranded binding protein Viral Proteins Viral entry Virology Viral structural protein medicine Viral shedding DNA Primers Nuclease Deoxyribonucleases Virulence Genome Replication and Regulation of Viral Gene Expression Herpes simplex virus Viral replication Insect Science Mutation biology.protein |
| Popis: | Enzyme-dead mutations in the herpes simplex virus 1 UL12 gene that abolished its endo- and exonuclease activities only slightly reduced viral replication in cell cultures. However, the UL12 null mutation significantly reduced viral replication, suggesting that a UL12 function(s) unrelated to its nuclease activities played a major role in viral replication. In contrast, the enzyme-dead mutations significantly reduced viral neurovirulence in mice, suggesting that UL12 nuclease activities were critical for viral pathogenesis in vivo . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|