Cyclo(Phe-Pro) Modulates the Expression of ompU in Vibrio spp
| Autor: | Chang-Ho Baek, In Hwang Kim, Dae-Kyun Park, Sang-Ho Choi, Byung-Soo Kim, Kun-Soo Kim, Ji-Hyoun Kwon, Ko-Eun Lee, Kyu-Ho Lee, Won Koo Lee |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cell signaling
Sodium Mutant chemistry.chemical_element Vibrio vulnificus Microbial Communities and Interactions complex mixtures Models Biological Peptides Cyclic Microbiology Molecular Biology Gene Vibrio biology Dipeptides bacterial infections and mycoses biology.organism_classification Adaptation Physiological Molecular biology chemistry Stationary phase Cell culture bacteria Signal Transduction |
| Zdroj: | Journal of Bacteriology. 188:2214-2221 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.188.6.2214-2221.2006 |
| Popis: | Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1 H nuclear magnetic resonance analyses indicated that the compound was cyclo( l -Phe- l -Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR -null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae , V. parahaemolyticus , and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|